Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Distinguishing Between Isobaric Ions Using Microdroplet Hydrogen-Deuterium Exchange Mass Spectrometry

Version 1 : Received: 1 October 2021 / Approved: 4 October 2021 / Online: 4 October 2021 (09:21:39 CEST)

A peer-reviewed article of this Preprint also exists.

Song, X.; Li, J.; Mofidfar, M.; Zare, R.N. Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry. Metabolites 2021, 11, 728. Song, X.; Li, J.; Mofidfar, M.; Zare, R.N. Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry. Metabolites 2021, 11, 728.

Journal reference: Metabolites 2021, 11, 728
DOI: 10.3390/metabo11110728

Abstract

Isobaric ions having the same mass-to-charge ratio cannot be separately identified by mass spectrometry (MS) alone, but this limitation can be overcome using hydrogen-deuterium exchange (HDX) in microdroplets. Because isobaric ions may contain a varied number of exchangeable sites and different types of functional groups, each one produces a unique MS spectral pattern after droplet spray HDX without the need for MS/MS experiments or introduction of ion mobility measurements. As an example of the power of this approach, isobaric ions in urinary metabolic profiles are identified and used to distinguish between healthy individuals and those having bladder cancer.

Keywords

hydrogen-deuterium exchange; mass spectrometry; isobaric ions; ambient ionization; metabolic profiling

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