Song, X.; Li, J.; Mofidfar, M.; Zare, R.N. Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry. Metabolites2021, 11, 728.
Song, X.; Li, J.; Mofidfar, M.; Zare, R.N. Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry. Metabolites 2021, 11, 728.
Isobaric ions having the same mass-to-charge ratio cannot be separately identified by mass spectrometry (MS) alone, but this limitation can be overcome using hydrogen-deuterium exchange (HDX) in microdroplets. Because isobaric ions may contain a varied number of exchangeable sites and different types of functional groups, each one produces a unique MS spectral pattern after droplet spray HDX without the need for MS/MS experiments or introduction of ion mobility measurements. As an example of the power of this approach, isobaric ions in urinary metabolic profiles are identified and used to distinguish between healthy individuals and those having bladder cancer.
hydrogen-deuterium exchange; mass spectrometry; isobaric ions; ambient ionization; metabolic profiling
CHEMISTRY, Analytical Chemistry
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