Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

In-vitro Investigation of Polymeric Lipid Hybrid Nanoparticles (Plhns) Of a Chemotherapeutic Drug for the Treatment of Glioblastoma Multiforme

Version 1 : Received: 19 July 2021 / Approved: 21 July 2021 / Online: 21 July 2021 (16:37:21 CEST)

How to cite: Bhattacharya, S. In-vitro Investigation of Polymeric Lipid Hybrid Nanoparticles (Plhns) Of a Chemotherapeutic Drug for the Treatment of Glioblastoma Multiforme. Preprints 2021, 2021070503 (doi: 10.20944/preprints202107.0503.v1). Bhattacharya, S. In-vitro Investigation of Polymeric Lipid Hybrid Nanoparticles (Plhns) Of a Chemotherapeutic Drug for the Treatment of Glioblastoma Multiforme. Preprints 2021, 2021070503 (doi: 10.20944/preprints202107.0503.v1).

Abstract

PHLNs (polymeric lipid hybrid nanoparticles) are core–shell nanoparticle structures made up of polymer cores and lipid shells that have properties similar to both polymeric nanoparticles and liposomes. Methotrexate (MTX) loaded PLHNPs containing tween 80, phosphatidylcholine, poly D, L-lactic-co-glycolic acid (PLGA) & glyceryl tripalmitate prepared using solvent injection & homogenization method for glioblastoma treatment option. The MTX loaded PLHNPs optimized by Box–Behnken design to minimize particle size, higher entrapment efficacy, and maximize MTX concentration in the brain at 4h. The particle size, entrapment efficacy, concentration of drug in brain at 4h, zeta potential and AUC(Brain)/AUC(Plasma) ratio were in the range of 173.51-233.37nm, 70.56-86.34%, 6.38-12.38 μg/mL, 25.78-36.31mV & 1.02-5.32. in-vitro drug release studies, cellular internalization of optimized formulation against U-87 MG shows good anticancer effects.

Subject Areas

Cytotoxicity; Glioblastoma multiform; Lactate dehydrogenase (LDH); Poly D- L-lactic-co-glycolic acid (PLGA); U-87 MG glioma cell lines

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