Version 1
: Received: 17 July 2021 / Approved: 21 July 2021 / Online: 21 July 2021 (15:43:11 CEST)
How to cite:
Yasuma, T.; Toda, M.; Kobori, H.; Tada, N.; D'Alessandro-Gabazza, C.; Gabazza, E. Subcritical Water Extracts from Agaricus blazei Murrill's Mycelium Inhibits the Expression of Immune Checkpoint Molecules and Axl receptor. Preprints2021, 2021070502. https://doi.org/10.20944/preprints202107.0502.v1
Yasuma, T.; Toda, M.; Kobori, H.; Tada, N.; D'Alessandro-Gabazza, C.; Gabazza, E. Subcritical Water Extracts from Agaricus blazei Murrill's Mycelium Inhibits the Expression of Immune Checkpoint Molecules and Axl receptor. Preprints 2021, 2021070502. https://doi.org/10.20944/preprints202107.0502.v1
Yasuma, T.; Toda, M.; Kobori, H.; Tada, N.; D'Alessandro-Gabazza, C.; Gabazza, E. Subcritical Water Extracts from Agaricus blazei Murrill's Mycelium Inhibits the Expression of Immune Checkpoint Molecules and Axl receptor. Preprints2021, 2021070502. https://doi.org/10.20944/preprints202107.0502.v1
APA Style
Yasuma, T., Toda, M., Kobori, H., Tada, N., D'Alessandro-Gabazza, C., & Gabazza, E. (2021). Subcritical Water Extracts from <em>Agaricus blazei</em> Murrill's Mycelium Inhibits the Expression of Immune Checkpoint Molecules and Axl receptor. Preprints. https://doi.org/10.20944/preprints202107.0502.v1
Chicago/Turabian Style
Yasuma, T., Corina D'Alessandro-Gabazza and Esteban Gabazza. 2021 "Subcritical Water Extracts from <em>Agaricus blazei</em> Murrill's Mycelium Inhibits the Expression of Immune Checkpoint Molecules and Axl receptor" Preprints. https://doi.org/10.20944/preprints202107.0502.v1
Abstract
Agaricus blazei Murrill or Himematsutake is an edible and medicinal mushroom. Agaricus blazei Murrill's fruiting body extracts have anticancer properties, although the mechanism is unknown. Basic or organic solvents, which are hazardous for human health, are generally used to prepare Agaricus blazei Murrill's extracts. Inhibition of immune checkpoint molecules and Axl receptor is an effective therapy in cancer. This study assessed whether subcritical water extracts of the Agaricus blazei Murrill's fruiting body or mycelium affect the expression of Axl and immune checkpoint molecules in lung cancer cells. We used A549 cells and mouse bone marrow-derived dendritic cells in the experiments. We prepared subcritical water extracts from the Agaricus blazei Murrill's fruiting body or mycelium. The subcritical water extracts from the Agaricus blazei Murrill's fruiting body or mycelium significantly inhibited the expression of immune checkpoint molecules and Axl compared to saline-treated cells. Also, the hot water extract, subcritical water extract, and the hot water extraction residue subcritical water extract from the Agaricus blazei Murrill's mycelium significantly enhanced the expression of maturation markers in dendritic cells. These observations suggest that the subcritical water extract from Agaricus blazei Murrill's mycelium is a promising therapeutic tool for stimulating the immune response in cancer.
Biology and Life Sciences, Agricultural Science and Agronomy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
