Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Isolation and Characterization of anti-Streptococcus Mutans Phage as a Possible Treatment Agent for Caries

Version 1 : Received: 17 March 2021 / Approved: 2 April 2021 / Online: 2 April 2021 (11:15:54 CEST)

How to cite: Ben Zaken, H.; Kraitman, R.; Coppenhagen-Glazer, S.; Khalifa, L.; Alkalay-Oren, S.; Ben-Gal, G.; Beyth, N.; Hazan, R. Isolation and Characterization of anti-Streptococcus Mutans Phage as a Possible Treatment Agent for Caries . Preprints 2021, 2021040045 (doi: 10.20944/preprints202104.0045.v1). Ben Zaken, H.; Kraitman, R.; Coppenhagen-Glazer, S.; Khalifa, L.; Alkalay-Oren, S.; Ben-Gal, G.; Beyth, N.; Hazan, R. Isolation and Characterization of anti-Streptococcus Mutans Phage as a Possible Treatment Agent for Caries . Preprints 2021, 2021040045 (doi: 10.20944/preprints202104.0045.v1).

Abstract

Streptococcus mutans is a key bacterium in dental caries- one of the most prevalent chronic infectious diseases. Conventional treatment both fails to specifically target the pathogenic bacteria and at-tempts to eradicate commensal bacteria as well. Thus, caries remains one of most common and challenging diseases. The use of bacterial viruses as anti-bacterial agents, is gaining interest worldwide. Hardly any phages were described against S. mutans. The objective of this study was to isolate anti-S. mutans phages and to characterize their antimi-crobial properties. Human saliva samples were filtered and screened for potential phages. Standard double-layered agar method was used for isolation. Whole genome sequence analysis and morphology visualization by TEM, were used for anti-S. mutans phage identification. Antibacterial properties were evaluated using clinical strains and ATCC strains of S. mutans in various states. Antibacterial effect was also tested on human cariogenic dentin. One phage against S. mutans was isolated and termed SMHBZ8. This phage showed effective lytic activity in vitro against both planktonic and biofilm S. mutans cultures. Moreover, the phage showed antibacterial effect when used on cariogenic dentin. The isolation and characterization of SMHBZ8 may be the first step in developing a potential phage therapy for dental caries.

Subject Areas

S. mutans; Phage therapy; Dental caries; S. mutans phage; Bacteriophage; Biofilm.

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