Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

A Computational Survey of The Intronic Micrornas in the X Chromosomes of Human and Chimpanzee, Their Target Gene Interactions and Its Impact on Gene Regulation

Version 1 : Received: 23 December 2020 / Approved: 24 December 2020 / Online: 24 December 2020 (15:30:33 CET)

How to cite: Mallavarpu Ambrose, J.; Daniel, A.A.; Kullappan, M.; Hussain, S.; James, K.M.; Sreekandan, R.N.; Deiva Suga, S.S.; Kamaraj, D.; Veera Raghavan, V.P.; Surapaneni, K.M. A Computational Survey of The Intronic Micrornas in the X Chromosomes of Human and Chimpanzee, Their Target Gene Interactions and Its Impact on Gene Regulation. Preprints 2020, 2020120637 (doi: 10.20944/preprints202012.0637.v1). Mallavarpu Ambrose, J.; Daniel, A.A.; Kullappan, M.; Hussain, S.; James, K.M.; Sreekandan, R.N.; Deiva Suga, S.S.; Kamaraj, D.; Veera Raghavan, V.P.; Surapaneni, K.M. A Computational Survey of The Intronic Micrornas in the X Chromosomes of Human and Chimpanzee, Their Target Gene Interactions and Its Impact on Gene Regulation. Preprints 2020, 2020120637 (doi: 10.20944/preprints202012.0637.v1).

Abstract

The knowledge of what separates us genetically from our less-evolved relatives is crucial for gaining new biomedical insights about the human-chimpanzee relatedness for the use of appropriate stand-in towards the development of new treatments and diagnostic aids for various ailments. Although the genomes of humans and chimpanzees share 99% similarity, significant differences exist between the two species in their non-coding intronic regions. However, no work has been carried out in the aspects of target prediction concerning the ‘predicted homology’ in their microRNA sequences. Non-coding miRNAs which are post-transcriptional regulators of development, differentiation, growth, and metabolism, harboring the intronic regions may be crucial for expanding the horizons of our understanding. In this study, we proposed to perform the target prediction for the human-chimp miRNA homologs in the PHEX gene of the human X chromosome using various computational tools and databases. We identified a total of 1296 human miRNAs, 46, 957 gene targets, and 30, 563 targets of human and homologous chimp miRNAs respectively. Furthermore, we analysed gene interacting networks to identify the top interacting targets in both the species. Finally, we interpreted the biological importance of top-interacting miRNAs and their targets. The results demonstrated varying levels of multiplicity and cooperativity between the predicted miRNAs and target genes in the two genera. Such miRNAs may be responsible for the dysregulation of gene expression in several signaling pathways.

Subject Areas

miRNA; gene targets; intronic miRNA; miRNA prediction; human miRNAs; PHEX miRNAs; chimpanzee homologues; experimentally-validated miRNA targets; miRNA computational survey; miRNA target multiplicity

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