Working Paper Article Version 1 This version is not peer-reviewed

Treatment by Cefotaxime with High-Fructose Diet Inducing Nonalcoholic Fatty Liver Disease and Gut Microbiota Dysbiosis in Mice

Version 1 : Received: 23 December 2020 / Approved: 24 December 2020 / Online: 24 December 2020 (09:23:32 CET)

How to cite: Lee, Y.; Chiu, C.; Chen, Y.; Huang, W.; Wang, Y.; Chiu, C.; Lin, T.; Hung, S.; Liu, J.; Chuang, H. Treatment by Cefotaxime with High-Fructose Diet Inducing Nonalcoholic Fatty Liver Disease and Gut Microbiota Dysbiosis in Mice. Preprints 2020, 2020120610 Lee, Y.; Chiu, C.; Chen, Y.; Huang, W.; Wang, Y.; Chiu, C.; Lin, T.; Hung, S.; Liu, J.; Chuang, H. Treatment by Cefotaxime with High-Fructose Diet Inducing Nonalcoholic Fatty Liver Disease and Gut Microbiota Dysbiosis in Mice. Preprints 2020, 2020120610

Abstract

High-fructose diet is the main cause of the metabolic syndrome and induces host gut microbial dysbiosis and related obesity and nonalcoholic fatty liver disease (NAFLD). Several antibiotic treatments could prevent endotoxemia and fatty liver. However, other studies demonstrated that a high-fructose diet could affect the gut bacterial dysbiosis and induce fatty liver. This study was performed to partially modify the gut microbial composition by a single cefotaxime treatment, which might influence the fructose-induced NAFLD severity. Male C57BL/6J Narl mice were divided into four groups: vehicle/control diet (VE-CD, 5010 chow), vehicle/high-fructose diet (VE-FD, 30% fructose), antibiotic (cefotaxime (CF))/CD, and CF/FD (n = 8 in each group) (treatment for 16 weeks). The NAFLD-related symptoms, including body weight gain, hepatic steatosis severity, epididymal white adipose tissue hypertrophy, and insulin resistance occurrence, were observed only in the CF-FD group. The increased protein expression of hepatic lipogenesis was observed in the CF-FD group, but lipolysis protein expression was no difference. CF-FD exhibited significantly reduced microbial diversity and microbial composition. Increased abundances of Lachnospiraceae and Clostridiales XIII were observed in the feces of CF-FD, compared to VE-FD. This novel model reveals that particular antibiotics such as cefotaxime may affect the gut microbiota exacerbating the hepatic steatosis by the high-fructose diet.

Subject Areas

High-fructose diet; nonalcoholic fatty liver disease; cefotaxime; gut microbiota dysbiosis

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