Saif, R.; Henkel, J.; Mahmood, T.; Ejaz, A.; Ahmed, F.; Zia, S. Detection of Whole Genome Selection Signatures of Pakistani Teddy Goat. Preprints2020, 2020120421. https://doi.org/10.20944/preprints202012.0421.v1
APA Style
Saif, R., Henkel, J., Mahmood, T., Ejaz, A., Ahmed, F., & Zia, S. (2020). <strong>Detection of Whole Genome Selection Signatures of Pakistani Teddy Goat</strong>. Preprints. https://doi.org/10.20944/preprints202012.0421.v1
Chicago/Turabian Style
Saif, R., Fraz Ahmed and Saeeda Zia. 2020 "<strong>Detection of Whole Genome Selection Signatures of Pakistani Teddy Goat</strong>" Preprints. https://doi.org/10.20944/preprints202012.0421.v1
Abstract
Whole genome pooled sequence data of 12 Pakistani Teddy goats is analyzed for positive selection signatures as their breed defining characteristics. Selection imprints left in the Teddy genome are unveiled by genomic differentiation after the successful paired-end alignment of 635,357,043 reads with (ARS1) reference genome assembly. Pooled-heterozygosity ( ) and Tajima’s D (TD) are applied for validation and getting better hits of selection signals, while pairwise FST statistics is conducted on Teddy vs. Bezoar (wild goat ancestor) for genomic differentiation. Annotation of regions under positive selection reveals 59 genes underlying production and adaptive traits. score ≥ 5 detected six windows having highest scores on Chr. 29, 9, 25, 15 and 14 that harbor HRASLS5, LACE1 and AXIN1 genes which are candidate for embryonic development, lactation and body height. Secondly, TD value of ≤ -2.2 showed 4 windows with very strong hits on Chr.5 & 9 harbor STIM1 and ADM genes related to body mass and weight. Lastly, FST analysis generated three strong signals with threshold ≤ 0.42 on Chr.12 & 5 harbor ITGB1 gene associated with milk production & lactation traits. Other significant selection signatures encompass genes associated with wool production, prolificacy, immunity and coat colors. In brief, this study identified the genes under selection in this Pakistani goat breed that will be helpful to refining future breeding policies and converging required productive traits within and across other goat breeds and to explore full genetic potential of this valued livestock species.
Biology and Life Sciences, Biology and Biotechnology
Copyright:
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