Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Isolation and Identification of Gram Positive and Negative Bacteria from Dairy Department Waste Lines at Baraton University

Version 1 : Received: 10 November 2020 / Approved: 25 November 2020 / Online: 25 November 2020 (08:36:32 CET)

How to cite: Wanyama, S. Isolation and Identification of Gram Positive and Negative Bacteria from Dairy Department Waste Lines at Baraton University. Preprints 2020, 2020110626 (doi: 10.20944/preprints202011.0626.v1). Wanyama, S. Isolation and Identification of Gram Positive and Negative Bacteria from Dairy Department Waste Lines at Baraton University. Preprints 2020, 2020110626 (doi: 10.20944/preprints202011.0626.v1).

Abstract

AbstractBaraton University dairy farm is an environment that attracts a microbiologist to inquire the composition of bacteria that exist there in. The knowledge of bacteria has in time and again amazed the life scientist community that have invested to acquire more information in this microbiology world.The study engages fundamental tests such as gram stain, endospore stain, and assays for specific microbial activities & enzymes, susceptibility on disinfectant and antibiotic, utilization of specific substrate and culture characteristics. The two organisms (gram negative and positive) tested positive for sucrose & lactose fermentation, Indole & Methly red, catalase & Oxidase, were both facultative and motile. On contrary, gram positive bacteria had spores and had a gamma haemolysis on Blood Agar, while gram negative bacteria haemolysed beta haemolysis. To draw a conclusion on the identity of the two organisms is that, the gram positive is a Bacillus ........ while gram negative is Escherichia coli.

Subject Areas

Isolation; Identification; gram positive; gram negative; tests staining; microbio metabolic activities

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