Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Rapid Hormetic Responses of Photosystem II Photochemistry to Cadmium Exposure

Version 1 : Received: 8 November 2020 / Approved: 9 November 2020 / Online: 9 November 2020 (16:06:01 CET)

How to cite: Adamakis, I.S.; Sperdouli, I.; Hanć, A.; Dobrikova, A.; Apostolova, E.; Moustakas, M. Rapid Hormetic Responses of Photosystem II Photochemistry to Cadmium Exposure. Preprints 2020, 2020110283 (doi: 10.20944/preprints202011.0283.v1). Adamakis, I.S.; Sperdouli, I.; Hanć, A.; Dobrikova, A.; Apostolova, E.; Moustakas, M. Rapid Hormetic Responses of Photosystem II Photochemistry to Cadmium Exposure. Preprints 2020, 2020110283 (doi: 10.20944/preprints202011.0283.v1).

Abstract

Five-day exposure of clary sage (Salvia sclarea) to 100 μM cadmium (Cd) in hydroponics was sufficient to increase Cd concentrations significantly in roots and aboveground parts and affect negatively whole plant levels of calcium (Ca) and magnesium (Mg), since Cd competes for Ca channels, while reduced Mg concentrations are associated with increased Cd tolerance. Total zinc (Zn), copper (Cu), and iron (Fe) uptake increased but their translocation to the aboveground parts decreased possible due to translocation barriers. Despite the substantial levels of Cd in leaves, without any observed defects on chloroplast ultrastructure, an enhanced photosystem II (PSII) efficiency was observed, with a higher fraction of absorbed light energy to be directed to photochemistry (ΦPSΙΙ). The concomitant increase in the photoprotective mechanism of non-photochemical quenching of photosynthesis (NPQ) resulted in an important decrease in the dissipated non-regulated energy (ΦNO), modifying the homeostasis of reactive oxygen species (ROS), through a decreased singlet oxygen (1O2) formation. Thus, when clary sage was exposed to Cd for a short period, tolerance mechanisms were triggered, with PSII photochemistry to be regulated by NPQ in such a way that PSII efficiency to be enhanced. However, exposure to a combination of Cd and high light or for longer duration (8 days) to Cd alone, resulted in an inhibition of PSII functionality pointing out towards Cd toxicity. Thus, the rapid activation of PSII functionality at short time exposures and the inhibition at longer duration suggests a hormetic response and describes these effects in terms of “adaptive response” and “toxicity”, respectively.

Subject Areas

Salvia sclarea; chlorophyll fluorescence imaging; non-photochemical quenching; tolerance mechanism; photoprotective mechanism; photosynthesis; reactive oxygen species; toxicity; adaptive response, photochemical efficiency

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.