Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

PNA-based Graphene Oxide/porous Silicon Hybrid Biosensor: Towards a Label-free Optical Assay for Brugada Syndrome

Version 1 : Received: 29 October 2020 / Approved: 2 November 2020 / Online: 2 November 2020 (11:12:24 CET)

A peer-reviewed article of this Preprint also exists.

Moretta, R.; Terracciano, M.; Borbone, N.; Oliviero, G.; Schiattarella, C.; Piccialli, G.; Falanga, A.P.; Marzano, M.; Dardano, P.; De Stefano, L.; Rea, I. PNA-Based Graphene Oxide/Porous Silicon Hybrid Biosensor: Towards a Label-Free Optical Assay for Brugada Syndrome. Nanomaterials 2020, 10, 2233. Moretta, R.; Terracciano, M.; Borbone, N.; Oliviero, G.; Schiattarella, C.; Piccialli, G.; Falanga, A.P.; Marzano, M.; Dardano, P.; De Stefano, L.; Rea, I. PNA-Based Graphene Oxide/Porous Silicon Hybrid Biosensor: Towards a Label-Free Optical Assay for Brugada Syndrome. Nanomaterials 2020, 10, 2233.

Journal reference: Nanomaterials 2020, 10, 2233
DOI: 10.3390/nano10112233

Abstract

Peptide nucleic acid (PNA) is a synthetic DNA mimic that outperforms the properties of traditional oligonucleotides (ONs). On account of its outstanding features, such as remarkable binding affinity towards complementary DNA or RNA as well as high thermal and chemical stability, PNA has been proposed as a valuable alternative to the ON probe in gene-sensor design. In this study, a hybrid transducer made-up of graphene oxide (GO) nano-sheets covalently grafted onto a porous silicon (PSi) matrix has been investigated for the early detection of a genetic cardiac disorder, the Brugada syndrome (BS). A functionalization strategy towards the realization of a potential PNA-based device is described. A peptide nucleic acid (PNA), able to detect the SCN5A associated with the BS has been properly synthesized and used as a bioprobe for the realization of a proof-of-concept label-free optical PNA-biosensor. PSi reflectance and GO photoluminescence (PL) signals were simultaneously exploited for the monitoring of the device functionalization and response.

Subject Areas

Brugada syndrome; peptide nucleic acid-PNA, porous silicon; graphene oxide; optical biosensor; hybrid device

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