Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

The Impact of Sex on Changes in Plasma Corticosterone and Cotinine Levels Induced by Nicotine in C57BL/6J Mice

Version 1 : Received: 6 September 2020 / Approved: 7 September 2020 / Online: 7 September 2020 (08:08:54 CEST)

How to cite: Nguyen, K.; Kanamori, K.; Hamid, A.; Lutfy, K. The Impact of Sex on Changes in Plasma Corticosterone and Cotinine Levels Induced by Nicotine in C57BL/6J Mice. Preprints 2020, 2020090159 (doi: 10.20944/preprints202009.0159.v1). Nguyen, K.; Kanamori, K.; Hamid, A.; Lutfy, K. The Impact of Sex on Changes in Plasma Corticosterone and Cotinine Levels Induced by Nicotine in C57BL/6J Mice. Preprints 2020, 2020090159 (doi: 10.20944/preprints202009.0159.v1).

Abstract

We assessed if there were any sex-related differences in the ability of nicotine to increase plasma corticosterone secretion after single or repeated nicotine administration. For single-dose studies, male and female mice were habituated to the test room for 1 h and injected with saline or nicotine (0.25 or 1 mg/kg, s.c.). In repeated-dosing studies, mice were injected with saline or nicotine (1 mg/kg, s.c.) once daily for six days, and, on day 7, received nicotine (1 mg/kg, s.c.). The mice were euthanized 15 min later, and trunk blood was collected for the measurement of corticosterone, nicotine, and cotinine. Our results showed that saline or nicotine each significantly increased plasma corticosterone levels in both male and female mice, with a greater response in female mice. Plasma corticosterone levels were increased in male but not female mice after repeated compared to single nicotine administration. The level of cotinine, a biomarker of nicotine use, was significantly higher in female than in male mice. Taken together, these novel findings suggest that female mice responded to nicotine and stress of handling more than male mice and provide for the first-time quantitative data on the male-female differences in nicotine-induced elevations of corticosterone and of cotinine.

Subject Areas

Nicotine; Cotinine; Corticosterone; LCMS; Sex; C57BL/6J mouse; CYP2A5; Plasma Levels; Subcutaneous Injection

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