Preprint Communication Version 1 Preserved in Portico This version is not peer-reviewed

Bioguided Fractionation of Hypoglycaemic Component in Methanol Extract of Vernonia amygdalina: An In Vivo Study

Stanley Irobekhian Reuben Okoduwa
* ORCID logo ,
Isma'ila A. Umar
,
Dorcas B. James
,
Hajiya M. Inuwa
,
James D. Habila
,
Alessandro Venditti
Version 1 : Received: 26 July 2020 / Approved: 27 July 2020 / Online: 27 July 2020 (00:32:27 CEST)

A peer-reviewed article of this Preprint also exists.

Okoduwa S.I.R., Umar I.A., James D.B., Inuwa H.M., Habila J.D. & Venditti A (2020). Bioguided fractionation of hypoglycaemic component in methanol extract of Vernonia amygdalina: an in vivo study. Natural Products Research. 13 Aug 2020. https://doi.org/10.1080/14786419.2020.1805605 Okoduwa S.I.R., Umar I.A., James D.B., Inuwa H.M., Habila J.D. & Venditti A (2020). Bioguided fractionation of hypoglycaemic component in methanol extract of Vernonia amygdalina: an in vivo study. Natural Products Research. 13 Aug 2020. https://doi.org/10.1080/14786419.2020.1805605

Abstract

Nine components (C1-C9) were isolated from chloroform fraction of fractionated methanol extracts of Vernonia amygdalina leaves (FMEVA) by column chromatography. All the components C1 to C9 were purified and screened for hypoglycaemic activities in type-2 diabetic rats. The most potent hypoglycaemic component was elucidated on the basis of extensive spectroscopic (1D-, 2D-NMR, GC-MS, FTIR) data analysis. The Component C5 was found to be the most potent hypoglycaemic in reducing blood glucose by 12.55 ± 3.55% at 4 h post-oral administration, when compared to the positive (18.07 ± 1.20%) and negative (-1.99 ± 0.43%) controls. The spectroscopic data analysis reveals that the isolated compound has a structure consistent with 11β,13-dihydrovernolide. The isolated compound is part of the hypoglycaemic components present in V. amygdalina leaves that is responsible for the anti-diabetic activities. Further research is needed in the development of this compound or its derivatives for pharmaceutical use.

Keywords

Anti-diabetic; hyperglycaemia; hypoglycaemic; Vernonia amygdalina; Type-2 diabetes

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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