Preprint Article Version 1 This version is not peer-reviewed

Molecular, Sequencing and Bioinformatics of Insulin- like Growth Factor 1(IGF-1) Gene and Transforming Growth Factor β2 Gene Associations with Growth Traits in Three Strains of Chicken

Version 1 : Received: 12 July 2020 / Approved: 14 July 2020 / Online: 14 July 2020 (05:17:40 CEST)

How to cite: El-Tahawy, W.; Abdel-Rahman, M. Molecular, Sequencing and Bioinformatics of Insulin- like Growth Factor 1(IGF-1) Gene and Transforming Growth Factor β2 Gene Associations with Growth Traits in Three Strains of Chicken. Preprints 2020, 2020070289 (doi: 10.20944/preprints202007.0289.v1). El-Tahawy, W.; Abdel-Rahman, M. Molecular, Sequencing and Bioinformatics of Insulin- like Growth Factor 1(IGF-1) Gene and Transforming Growth Factor β2 Gene Associations with Growth Traits in Three Strains of Chicken. Preprints 2020, 2020070289 (doi: 10.20944/preprints202007.0289.v1).

Abstract

Molecular analysis is an easier means to identify and isolate a specific gene which has imperative function for growth, body composition , fat deposition, metabolic and skeletal traits as well as the molecular genetics selection on individual genes is a very efficient method to genetically improve economically important traits in chickens. Insulin- like growth factor 1 ( IGF-1)is a member of a heterogeneous group of peptides with important growth.Transforming growth factorβ ( TGF-β) belongs to a large family of growth and differentiation factors that play a pivotal role in a great variety of biological activities including morphogenesis, development and differentiation. DNA was extracted from 48 chickens sampled from three strains Lohman (17) , Sinai (24) and Gimmizah (7) IGF-1 gene and TGFβ2 gene were amplified using PCR protocol. Electrophoresis was carried out on the products of PCR , bands viewed on transilluminator. The size of IGF-1 gene was 675 bp while the size of TGFβ2 gene was 188bp. Sharp bands were purified and sequenced and used the dendrogram to show the relationships between other vertebrate species

Subject Areas

chickens; IGF-1 gene; TGFβ2 gene; DNA extraction; purification; sequencing and bioinformatics

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