Version 1
: Received: 23 March 2020 / Approved: 24 March 2020 / Online: 24 March 2020 (08:41:53 CET)
How to cite:
Patri, M. Neuropeptide Y Expression Confers Benzo[a]pyrene Induced DNA Damage and Microtubule Disruption in Human Neuroblastoma SH-SY5Y Cells. Preprints2020, 2020030357. https://doi.org/10.20944/preprints202003.0357.v1
Patri, M. Neuropeptide Y Expression Confers Benzo[a]pyrene Induced DNA Damage and Microtubule Disruption in Human Neuroblastoma SH-SY5Y Cells. Preprints 2020, 2020030357. https://doi.org/10.20944/preprints202003.0357.v1
Patri, M. Neuropeptide Y Expression Confers Benzo[a]pyrene Induced DNA Damage and Microtubule Disruption in Human Neuroblastoma SH-SY5Y Cells. Preprints2020, 2020030357. https://doi.org/10.20944/preprints202003.0357.v1
APA Style
Patri, M. (2020). Neuropeptide Y Expression Confers Benzo[a]pyrene Induced DNA Damage and Microtubule Disruption in Human Neuroblastoma SH-SY5Y Cells. Preprints. https://doi.org/10.20944/preprints202003.0357.v1
Chicago/Turabian Style
Patri, M. 2020 "Neuropeptide Y Expression Confers Benzo[a]pyrene Induced DNA Damage and Microtubule Disruption in Human Neuroblastoma SH-SY5Y Cells" Preprints. https://doi.org/10.20944/preprints202003.0357.v1
Abstract
Benzo[a]pyrene (B[a]P), is a family member of polycyclic aromatic hydrocarbons and a widespread environmental pollutant and neurotoxicant that contribute to the development of cancer. Microtubules are polymers of tubulin that form part of the cytoskeleton and target for anticancer drugs. Furthermore, NPY significantly increased the percentage of cells in S and G2/M phases. However, little is known about the specific role of NPY in proliferation and the underlying protective mechanism remains unclear. Hence, the aim of this work was to investigate the effect of B[a]P on SH-SY5Y neuroblastoma cells and to explore the potential mechanism for alteration of tubulin-microtubule equilibrium causing mitotic arrest and NPY expression. The present findings showed B[a]P treatment significantly increase number of SH-SY5Y cells in S and G2/M phase as compared to G1 phase and provokes cell cycle arrest that correlated with significant decrease in G0/G1 cells. Immunofluorescence study showed significantly distorted tubulin arrangement from metaphasic plate in formation of bipolar mitotic spindle apparatus. Further, higher doses of B[a]P treatment lead to chromosomal abnormalities accompanied by DNA damage due ROS causing oxidative stress showing significant decrease in tubulin protein around spindle. The results of present study demonstrated that NPY exerts a proliferative and protective effect on B[a]P-induced oxidative stress in a dose-dependent manner in vitro and importantly, these effects may be mediated via mitotic arrest and involved in spindle arrangement during cell division. Our findings addresses a novel pathological outcomes of B[a]P-induced NPY expression by oxidative stress through spindle abnormalities leading to microtubule disruption.
Keywords
Benzo[a]pyrene; Microtubule; Neuroblastoma; Cell cycle arrest, Neuropeptide Y
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
.png)
