Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Protective Effect of New Formulation of Carnosine+Hyaluronic Acid on the Inflammation and Cartilage Degradation in the Experimental Model of Osteoarthritis

Version 1 : Received: 10 January 2020 / Approved: 11 January 2020 / Online: 11 January 2020 (11:17:18 CET)

A peer-reviewed article of this Preprint also exists.

Siracusa, R.; Impellizzeri, D.; Cordaro, M.; Peritore, A.F.; Gugliandolo, E.; D’Amico, R.; Fusco, R.; Crupi, R.; Rizzarelli, E.; Cuzzocrea, S.; Vaccaro, S.; Pulicetta, M.; Greco, V.; Sciuto, S.; Schiavinato, A.; Messina, L.; Di Paola, R. The Protective Effect of New Carnosine-Hyaluronic Acid Conjugate on the Inflammation and Cartilage Degradation in the Experimental Model of Osteoarthritis. Appl. Sci. 2020, 10, 1324. Siracusa, R.; Impellizzeri, D.; Cordaro, M.; Peritore, A.F.; Gugliandolo, E.; D’Amico, R.; Fusco, R.; Crupi, R.; Rizzarelli, E.; Cuzzocrea, S.; Vaccaro, S.; Pulicetta, M.; Greco, V.; Sciuto, S.; Schiavinato, A.; Messina, L.; Di Paola, R. The Protective Effect of New Carnosine-Hyaluronic Acid Conjugate on the Inflammation and Cartilage Degradation in the Experimental Model of Osteoarthritis. Appl. Sci. 2020, 10, 1324.

Journal reference: Appl. Sci. 2020, 10, 1324
DOI: 10.3390/app10041324

Abstract

Osteoarthritis (OA) is a disease that currently has no cure. There are numerous studies showing that carnosine and hyaluronic acid (HA) have a positive pharmacological action during joint inflammation. For this reason, the goal of this research was to discover the protective effect of a new HA+Carnosine formulation (FidHycarn) on the inflammatory response and on the cartilage degradation in in vivo experimental model of OA. This model was induced by a single intra-articular (i.ar.) injection of 25µl normal saline having 1mg of monosodium iodoacetate solution (MIA) in the knee joint. MIA injection caused histological alterations and degradation of cartilage as well as behavioral changes. Oral treatment with FidHycarn ameliorated the macroscopic signs, improved thermal hyperalgesia and weight distribution of hind paw as well as decreased histological and radiographic alterations. The oxidative damage was analyzed by evaluating the levels of nitrotyrosine and inducible nitric oxide synthase (iNOS) that were significantly reduced in FidHycarn rats. Moreover, the levels of pro-inflammatory cytokines and chemokines were also significantly reduced by FidHycarn. However, interestingly, in more cases, the effects of FidHycarn were not statistically different to Naproxen used as positive control. Thus, the new formulation containing Carnosine and HA could represent an interesting therapeutic strategy to combat osteoarthritis.

Subject Areas

osteoarthritis; carnosine; hyaluronic acid; inflammation; oxidative stress

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