PreprintArticleVersion 1This version is not peer-reviewed
Saringosterol Acetate from a Popular Edible Seaweed, Hizikia Fusiformis Attenuated Proliferation of Human Lung Adenocarcinoma Epithelial Cells (A549 Cells) via Apoptosis Signaling Pathway
Version 1
: Received: 19 November 2019 / Approved: 20 November 2019 / Online: 20 November 2019 (11:19:19 CET)
How to cite:
Lee, J.; Ko, J.; Kim, C.Y.; Kim, Y.; Kim, J.; Jeon, Y. Saringosterol Acetate from a Popular Edible Seaweed, Hizikia Fusiformis Attenuated Proliferation of Human Lung Adenocarcinoma Epithelial Cells (A549 Cells) via Apoptosis Signaling Pathway. Preprints2019, 2019110239 (doi: 10.20944/preprints201911.0239.v1).
Lee, J.; Ko, J.; Kim, C.Y.; Kim, Y.; Kim, J.; Jeon, Y. Saringosterol Acetate from a Popular Edible Seaweed, Hizikia Fusiformis Attenuated Proliferation of Human Lung Adenocarcinoma Epithelial Cells (A549 Cells) via Apoptosis Signaling Pathway. Preprints 2019, 2019110239 (doi: 10.20944/preprints201911.0239.v1).
Cite as:
Lee, J.; Ko, J.; Kim, C.Y.; Kim, Y.; Kim, J.; Jeon, Y. Saringosterol Acetate from a Popular Edible Seaweed, Hizikia Fusiformis Attenuated Proliferation of Human Lung Adenocarcinoma Epithelial Cells (A549 Cells) via Apoptosis Signaling Pathway. Preprints2019, 2019110239 (doi: 10.20944/preprints201911.0239.v1).
Lee, J.; Ko, J.; Kim, C.Y.; Kim, Y.; Kim, J.; Jeon, Y. Saringosterol Acetate from a Popular Edible Seaweed, Hizikia Fusiformis Attenuated Proliferation of Human Lung Adenocarcinoma Epithelial Cells (A549 Cells) via Apoptosis Signaling Pathway. Preprints 2019, 2019110239 (doi: 10.20944/preprints201911.0239.v1).
Abstract
Hizikia fusiformis is a common, edible marine alga found in Asia. Although the anticancer activity of its extracts has previously been investigated, its active compounds have not been identified. In this study, saringosterol acetate (SA) was isolated from H. fusiformis extracts by centrifugal partition chromatography (CPC) system (two phase solvents condition: n-hexane:ethyl acetate/methanol:water = 5:3:7:1, v/v), exhibited anticancer effects in the human lung adenocarcinoma epithelial cell line, A549, by inducing apoptosis and sub-G1 phase cell cycle arrest. In addition, SA increased the expression of the pro-apoptotic proteins, Bax and cleaved caspase 3, and decreased that of the anti-apoptotic protein Bcl-xL. Although, SA did not affect the expression of p53, induces expression of Bid and caspase 8. In conclusion, we suggested that SA induces apoptosis against A549 cells via Bid and caspase 8 dependent pathway.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Ji-Hyeok Lee