Saringosterol Acetate from a Popular Edible Seaweed, Hizikia Fusiformis Attenuated Proliferation of Human Lung Adenocarcinoma Epithelial Cells (A549 Cells) via Apoptosis Signaling Pathway

Ji-Hyeok Lee; Ju-Young Ko; Chuk Young Kim; Yong-Tae Kim; Jaeil Kim; You-Jin Jeon

doi:10.20944/preprints201911.0239.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Version 1 : Received: 19 November 2019 / Approved: 20 November 2019 / Online: 20 November 2019 (11:19:19 CET)

Hizikia fusiformis is a common, edible marine alga found in Asia. Although the anticancer activity of its extracts has previously been investigated, its active compounds have not been identified. In this study, saringosterol acetate (SA) was isolated from H. fusiformis extracts by centrifugal partition chromatography (CPC) system (two phase solvents condition: n-hexane:ethyl acetate/methanol:water = 5:3:7:1, v/v), exhibited anticancer effects in the human lung adenocarcinoma epithelial cell line, A549, by inducing apoptosis and sub-G1 phase cell cycle arrest. In addition, SA increased the expression of the pro-apoptotic proteins, Bax and cleaved caspase 3, and decreased that of the anti-apoptotic protein Bcl-xL. Although, SA did not affect the expression of p53, induces expression of Bid and caspase 8. In conclusion, we suggested that SA induces apoptosis against A549 cells via Bid and caspase 8 dependent pathway.

edible marine alga; anticancer; hizikia fusiformis; saringosterol acetate; apoptosis

MEDICINE & PHARMACOLOGY, Pharmacology & Toxicology

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Saringosterol Acetate from a Popular Edible Seaweed, Hizikia Fusiformis Attenuated Proliferation of Human Lung Adenocarcinoma Epithelial Cells (A549 Cells) via Apoptosis Signaling Pathway

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