Working Paper Article Version 1 This version is not peer-reviewed

Differentially Gene Expression Analysis Using RNA-Seq In Purebred Sistani Cattle and Crossbred with Montbeliarde

Version 1 : Received: 1 October 2019 / Approved: 3 October 2019 / Online: 3 October 2019 (04:09:00 CEST)

How to cite: Esfeden, B.A.; Dashab, G.R.; Banabazi, M.H.; Rokouei, M. Differentially Gene Expression Analysis Using RNA-Seq In Purebred Sistani Cattle and Crossbred with Montbeliarde . Preprints 2019, 2019100035 Esfeden, B.A.; Dashab, G.R.; Banabazi, M.H.; Rokouei, M. Differentially Gene Expression Analysis Using RNA-Seq In Purebred Sistani Cattle and Crossbred with Montbeliarde . Preprints 2019, 2019100035

Abstract

Transcriptome study of the desired crossbreed and comparing it with pure parental population is one of the methods for evaluating and selecting cross with appropriate productivity, and yet disease resistant, high tolerance to environmental stresses and adapted to the climate of the breeding tract. In this study, RNA-seq technology was employed to detect differential expression of genes in purebred Sistani and Sistani × Montbeliard crossbreed. Blood samples were collected from four cows (two pure and two its crossbreed). Total RNA was extracted in Media Teb Gene Laboratory of Iran. After the treatment of mRNA, cDNA was synthesized. Quality control was performed with FastQC. Following the assembling of the transcripts with bovine reference genome, Cuffdiff was used to detect differentially gene expression in purebred and its crossbred. In total, 45 significantly differentially expressed genes were detected (false discovery rate q < 0.05) between purebred Sistani and Sistani × Montbeliard crossbreed. Gene Ontology enrichment and pathway analysis revealed that these differentially expressed genes were highly enriched in Inflammation mediated by chemokine and cytokine signaling pathways. These genes are reported to have role in immune response, calving, fertility, resistance to mastitis and also may have a role in different levels of heat tolerance and disease resistance. Therefore, Sistani × Montbeliard crossbred could be suitable for the climatic conditions of the Sistan region in Iran.

Subject Areas

differentially expressed genes; Sistani cow; crossbred; RNA sequencing

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.