Working Paper Article Version 1 This version is not peer-reviewed

Bioleaching of Chalcopyrite by Pure and Mixed Cultures at Low Temperature

Version 1 : Received: 29 September 2019 / Approved: 30 September 2019 / Online: 30 September 2019 (07:56:59 CEST)

How to cite: Peng, T.; Wang, J.; Miao, J.; Gu, G.; Qiu, G.; Dolgor, E.; Zeng, W. Bioleaching of Chalcopyrite by Pure and Mixed Cultures at Low Temperature. Preprints 2019, 2019090342 Peng, T.; Wang, J.; Miao, J.; Gu, G.; Qiu, G.; Dolgor, E.; Zeng, W. Bioleaching of Chalcopyrite by Pure and Mixed Cultures at Low Temperature. Preprints 2019, 2019090342

Abstract

Low-temperature biohydrometallurgy is implicated in metal recovery in alpine mining area, but our knowledge on its mechanism has been limited. To this end, an Acidithiobacillus ferrivorans strain and a mixed culture were used for chalcopyrite bioleaching at 6 °C. The passivation of the mineral surface was analyzed using X-ray photoelectron spectroscopy (XPS), the microbial community structure of the mixed culture-mediated system was tested using high-throughput sequencing technology, and the extracellular polymeric substances (EPS) and ferric iron on the microbe-mineral interface were measured. A higher copper extraction rate was achieved using the mixed culture but it did not relieve the passivation of the mineral surface. Acidithiobacillus spp. and Sulfobacillus spp. were the two major lineages in the mixed culture-mediated system. In the fast-leaching stage, more EPS and ferric iron were extracted in the mixed culture-mediated system. In conclusion, Sulfobacillus spp. can relieve the inhibition of organic components to Acidithiobacillus spp., maintaining the robustness of the leaching system and more Fe(III) complexed by elevated EPS can enhance chalcopyrite bioleaching by the mixed culture.

Subject Areas

chalcopyrite bioleaching; low temperature; microbial community; passivation; extracellular polymeric substances; ferric iron

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