Hu, B.; Yao, H.; Peng, X.; Wang, R.; Li, F.; Wang, Z.; Zhao, M.; Jin, L. Overexpression of Chalcone Synthase Improves Flavonoid Accumulation and Drought Tolerance in Tobacco. Preprints2019, 2019060103. https://doi.org/10.20944/preprints201906.0103.v1
Hu, B., Yao, H., Peng, X., Wang, R., Li, F., Wang, Z., Zhao, M., & Jin, L. (2019). Overexpression of Chalcone Synthase Improves Flavonoid Accumulation and Drought Tolerance in Tobacco. Preprints. https://doi.org/10.20944/preprints201906.0103.v1
Hu, B., Mingyue Zhao and Lifeng Jin. 2019 "Overexpression of Chalcone Synthase Improves Flavonoid Accumulation and Drought Tolerance in Tobacco" Preprints. https://doi.org/10.20944/preprints201906.0103.v1
Flavonoids are major secondary metabolites in plants, which play important roles in maintaining the cellular redox balance in cells. Chalcone synthase (CHS) is the key enzyme in the flavonoids biosynthesis pathway, and has been proved to monitor the changes to drought stress tolerance. In this work, we overexpressed a CHS gene in tobacco (Nicotiana tabacum). The transgenic tobacco plants were more tolerant than the control plants to drought stress. The transcription levels of the key genes involved in the flavonoids pathway and the contents of seven flavonoids were also significantly raised in the transgenic tobacco plants. In addition, overexpression of the CHS gene lead to a lower concentration of the oxidative stress product malondialdehyde. Overall, the NtCHS gene studied in this work was considered as a candidate gene for genetic engineering to enhance drought tolerance of plants and improve response to oxidative stress.
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