Preprint Article Version 1 This version is not peer-reviewed

Molecular Cloning and Expression Characterization of Three Suppressors of Cytokine Signaling Genes (SOCS5, SOCS6, SOCS7) from the Mealworm Beetle, Tenebrio molitor

Version 1 : Received: 24 January 2019 / Approved: 26 January 2019 / Online: 26 January 2019 (02:51:45 CET)

A peer-reviewed article of this Preprint also exists.

Patnaik, B.B.; Kim, B.B.; Jo, Y.H.; Bang, I.S. Molecular Cloning and Expression Analysis of Three Suppressors of Cytokine Signaling Genes (SOCS5, SOCS6, SOCS7) in the Mealworm Beetle Tenebrio molitor. Insects 2019, 10, 76. Patnaik, B.B.; Kim, B.B.; Jo, Y.H.; Bang, I.S. Molecular Cloning and Expression Analysis of Three Suppressors of Cytokine Signaling Genes (SOCS5, SOCS6, SOCS7) in the Mealworm Beetle Tenebrio molitor. Insects 2019, 10, 76.

Journal reference: Insects 2019, 10, 76
DOI: 10.3390/insects10030076

Abstract

Suppressors of cytokine signaling (SOCS) influence cytokine and growth factor signaling by negatively regulating the JAK-STAT pathway. This maintains homeostasis during host immune response. However, functional characterization of SOCS family members in invertebrates is limited. In this study, we discovered the Type-I subfamily of the SOCS genes in the mealworm beetle, T. molitor. The full-length ORFs of TmSOCS5, TmSOCS6, and TmSOCS7 consisted of 1,389, 897 and 1,458 nucleotides, encoding polypeptides of 462, 297 and 485 amino acids, respectively, The C-terminal region of TmSOCS was highly conserved in the SH2 and SOCS box domains. Phylogenetic analysis revealed that the three SOCS genes clustered within the same sub-family and the highest amino acid identity was with the Tribolium castaneum SOCS genes (TcSOCS). While the expression of TmSOCS5 and TmSOCS6 was low in larval, pupal, and adult stages of the insect, TmSOCS7 showed higher expression. The expression of TmSOCS5 and TmSOCS6 was higher in larval hemocytes and adult ovary. The microbes expressed the three TmSOCS genes to varying degrees. C. albicans elicited the strongest response in the host with highest 15-fold expression in TmSOCS7 3 h post-inoculation.  Collectively, these data suggest that the Type I TmSOCS could play a role in eliciting host immunity.

Subject Areas

Tenebrio molitor; suppressor of cytokine signaling; insect immunity; gene expression

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