Preprint Article Version 1 This version is not peer-reviewed

Interplay among calcium diet content, PTH(1-34) treatment and balance of bone homeostases in rat model: the trabecular bone as keystone

Version 1 : Received: 9 January 2019 / Approved: 10 January 2019 / Online: 10 January 2019 (11:38:42 CET)

How to cite: Ferretti, M.; Cavani, F.; Roli, L.; Checchi, M.; Magarò, M.S.; Bertacchini, J.; Palumbo, C. Interplay among calcium diet content, PTH(1-34) treatment and balance of bone homeostases in rat model: the trabecular bone as keystone. Preprints 2019, 2019010096 (doi: 10.20944/preprints201901.0096.v1). Ferretti, M.; Cavani, F.; Roli, L.; Checchi, M.; Magarò, M.S.; Bertacchini, J.; Palumbo, C. Interplay among calcium diet content, PTH(1-34) treatment and balance of bone homeostases in rat model: the trabecular bone as keystone. Preprints 2019, 2019010096 (doi: 10.20944/preprints201901.0096.v1).

Abstract

The present study is the second step (concerning the normal-diet restoration) of the our previous one (concerning the calcium-free diet) to determine whether the normal-diet restoration, with/without concomitant PTH(1-34) administration, can influence amounts and deposition sites of the total bone mass. Histomorphometric evaluations and immunohistochemical analysis for Sclerostin expression were conducted on the vertebral bodies and femurs  in rat model. The final goals are: i) to define timing and manners of bone mass changes when calcium is restored in the diet; ii) to analyze the different involvement of the two bony architectures having different metabolism (i.e. trabecular versus cortical bone); iii) to verify the eventual role of PTH(1-34) administration. Results evidenced the greater involvement of the trabecular bone with respect to the cortical one, in answering to different calcium diet content, and the effect of PTH mostly in the recovery of trabecular bony architecture. The main findings emerged from the present study are: i) the importance of the interplay between mineral homeostasis and skeletal homeostasis in modulating and guiding bone answers to dietary/metabolic alterations and ii) the evidence that the more involved bony architecture is the trabecular one, the most susceptible to the dynamical balance of the two homeostases.

Subject Areas

calcium diet content; mineral homeostasis; skeletal homeostasis; trabecular bone; PTH(1-34); rat

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