Preprint Article Version 1 This version is not peer-reviewed

Recovery of Active Polyphenol Oxidase and Peroxidase from Plant Tissues with High Phenolics and Chlorophylls

Version 1 : Received: 30 October 2018 / Approved: 1 November 2018 / Online: 1 November 2018 (18:00:20 CET)

A peer-reviewed article of this Preprint also exists.

Sommano SR. Recovery of Active Polyphenol Oxidase and Peroxidase from Plant Tissues with High Phenolics and Chlorophylls. SM Anal Bioanal Technique. 2018; 3(1): 1019. Sommano SR. Recovery of Active Polyphenol Oxidase and Peroxidase from Plant Tissues with High Phenolics and Chlorophylls. SM Anal Bioanal Technique. 2018; 3(1): 1019.

Journal reference: SM Anal Bioanal Technique 2018, 3, 1019
DOI: ISSN: 2573-3729

Abstract

The present protocol described extraction of active polyphenol oxidase and peroxidase from a plant rich in phenolics and chlorophylls in the post-harvest browning syndrome of B. myrtifolia.  Initially, general optimisation using conventional enzyme extractions was performed.  However, along with membrane-bound proteins, chlorophylls and phenols were also released with Triton X (TTX).  With a view to obtaining high enzymatic activity, removal of the released chlorophylls and phenols by formation of TTX-114 micelles in the detergent rich phase after high-temperature induced phase separation was tested.

Subject Areas

latent PPO; optimisation; peripheral membrane POD; total phenol content

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