Recovery of Active Polyphenol Oxidase and Peroxidase from Plant Tissues with High Phenolics and Chlorophylls

Sarana Rose Sommano

doi:10.20944/preprints201810.0758.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Version 1 : Received: 30 October 2018 / Approved: 1 November 2018 / Online: 1 November 2018 (18:00:20 CET)

The present protocol described extraction of active polyphenol oxidase and peroxidase from a plant rich in phenolics and chlorophylls in the post-harvest browning syndrome of B. myrtifolia. Initially, general optimisation using conventional enzyme extractions was performed. However, along with membrane-bound proteins, chlorophylls and phenols were also released with Triton X (TTX). With a view to obtaining high enzymatic activity, removal of the released chlorophylls and phenols by formation of TTX-114 micelles in the detergent rich phase after high-temperature induced phase separation was tested.

latent PPO; optimisation; peripheral membrane POD; total phenol content

BIOLOGY, Plant Sciences

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Recovery of Active Polyphenol Oxidase and Peroxidase from Plant Tissues with High Phenolics and Chlorophylls

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