Version 1
: Received: 16 October 2018 / Approved: 16 October 2018 / Online: 16 October 2018 (08:35:47 CEST)
How to cite:
Zhang, M.; Park, H.J.; Hong, K.; Park, C.; Song, H. Reproductive Toxicity of 4-Octylphenol Induced Mitochondria-Mediated Apoptosis in Male Mouse-Specific Niche Cells. Preprints2018, 2018100342. https://doi.org/10.20944/preprints201810.0342.v1
Zhang, M.; Park, H.J.; Hong, K.; Park, C.; Song, H. Reproductive Toxicity of 4-Octylphenol Induced Mitochondria-Mediated Apoptosis in Male Mouse-Specific Niche Cells. Preprints 2018, 2018100342. https://doi.org/10.20944/preprints201810.0342.v1
Zhang, M.; Park, H.J.; Hong, K.; Park, C.; Song, H. Reproductive Toxicity of 4-Octylphenol Induced Mitochondria-Mediated Apoptosis in Male Mouse-Specific Niche Cells. Preprints2018, 2018100342. https://doi.org/10.20944/preprints201810.0342.v1
APA Style
Zhang, M., Park, H.J., Hong, K., Park, C., & Song, H. (2018). Reproductive Toxicity of 4-Octylphenol Induced Mitochondria-Mediated Apoptosis in Male Mouse-Specific Niche Cells. Preprints. https://doi.org/10.20944/preprints201810.0342.v1
Chicago/Turabian Style
Zhang, M., Chankyu Park and Hyuk Song. 2018 "Reproductive Toxicity of 4-Octylphenol Induced Mitochondria-Mediated Apoptosis in Male Mouse-Specific Niche Cells" Preprints. https://doi.org/10.20944/preprints201810.0342.v1
Abstract
The toxic effects of 4-octylphenol (4-OP) have been studied in species such as mouse and fish; however, the toxic effects of 4-OP in male specific niche cells has not been researched. In this study, we investigated the molecular mechanism of toxicity of 4-OP in mouse TM4 Sertoli cells. TM4 cells were treated with four concentrations (0, 10, 30, and 50 µM/mL) of 4-OP at time points 24, 48, and 72 h. Cell viability and apoptosis assay was conducted following exposure. 4-OP significantly decreased cell viability in a concentration- and time-dependent manner, and increased apoptosis. Quantitative PCR analysis showed that Bad, Bax, and Bak mRNA expression levels were higher in exposed cells than in the control, but Bcl-2 expression was decreased. Western blotting revealed that 4-OP induced activities of caspase-3 and phosphorylation of Bad in a concentration- and time-dependent manner. Additionally, cytochrome C protein did not colocalize with mitochondria marker dye by 24 h. Cytochrome c protein expression increased in a time-dependent manner with 50 µM/mL. These results suggest that 4-OP induces mitochondria-mediated apoptosis by regulation of Bcl-2 family proteins and caspase-3 activation in male Sertoli cells.
Keywords
4-octylphenol; male sertoli cells; reproductive toxicity; apoptosis; mitochondria
Subject
Biology and Life Sciences, Anatomy and Physiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
