Preprint Article Version 1 This version is not peer-reviewed

Characterization of Human Chondrocytes from Less- vs. Severely-Affected Osteoarthritic Cartilage and Evaluation of their Ability to Develop into In Vitro 3D Models

Version 1 : Received: 12 October 2018 / Approved: 12 October 2018 / Online: 12 October 2018 (12:19:01 CEST)

How to cite: Binti Zakariah, N.N.; Bin Sulaiman, S.; Bin Yahaya, N.H.; Abdul Rani, R.; Binti Haji Idrus, R.; Roy Chowdhury, S. Characterization of Human Chondrocytes from Less- vs. Severely-Affected Osteoarthritic Cartilage and Evaluation of their Ability to Develop into In Vitro 3D Models. Preprints 2018, 2018100269 (doi: 10.20944/preprints201810.0269.v1). Binti Zakariah, N.N.; Bin Sulaiman, S.; Bin Yahaya, N.H.; Abdul Rani, R.; Binti Haji Idrus, R.; Roy Chowdhury, S. Characterization of Human Chondrocytes from Less- vs. Severely-Affected Osteoarthritic Cartilage and Evaluation of their Ability to Develop into In Vitro 3D Models. Preprints 2018, 2018100269 (doi: 10.20944/preprints201810.0269.v1).

Abstract

Osteoarthritis (OA) is a joint disease involving cartilage degeneration. This study aimed to compare properties of chondrocytes from less-affected (LA-Cartilage) and severely-affected (SA-Cartilage) of human OA articular cartilage. Based on Dougados classification, OA cartilage was classified into two groups; less-affected (Grade 0–1) and severely-affected (Grade 2–3). Chondrocytes from each group were cultured until passage (P) 4. Growth, migration, stem cell properties and chondrogenic properties under normal and inflammatory conditions, and the formation of in vitro 3D cartilage tissues were compared between groups. The growth and migratory properties of LA-chondrocytes and SA-chondrocytes were similar, except that the migration rate of SA-chondrocytes was significantly higher at P0 compared to LA-chondrocytes. Both LA-chondrocytes and SA-chondrocytes expressed mesenchymal stem cell markers and tri-lineage differentiation, but the expression of stem cell markers decreased significantly with increasing passage number. Exposure to inflammatory conditions induced distinct morphological changes and significant increases in expression of SOX9 at P4 and MMP3 at P1 for LA-chondrocytes. LA-chondrocytes and SA-chondrocytes able to develop into in vitro 3D constructs, but SA-chondrocytes exhibited superior cartilage-like properties. Chondrocytes from both less- and severely-affected regions are suitable to be used in clinical applications, however, chondrocytes from severely-affected regions could be a more favorable cell source.

Subject Areas

3D models; cartilage; chondrocytes; osteoarthritis (OA)

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