PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Inclusion of Antibodies to Cell Culture Media Preserves the Integrity of Genes Encoding RL13 and the Pentameric Complex Components during Fibroblast Passage of Human Cytomegalovirus
Version 1
: Received: 25 July 2018 / Approved: 26 July 2018 / Online: 26 July 2018 (04:31:39 CEST)
How to cite:
Ourahmane, A.; Cui, X.; He, L.; Dittmer, D.; Schleiss, M.; Hertel, L.; McVoy, M.A. Inclusion of Antibodies to Cell Culture Media Preserves the Integrity of Genes Encoding RL13 and the Pentameric Complex Components during Fibroblast Passage of Human Cytomegalovirus. Preprints2018, 2018070503. https://doi.org/10.20944/preprints201807.0503.v1
Ourahmane, A.; Cui, X.; He, L.; Dittmer, D.; Schleiss, M.; Hertel, L.; McVoy, M.A. Inclusion of Antibodies to Cell Culture Media Preserves the Integrity of Genes Encoding RL13 and the Pentameric Complex Components during Fibroblast Passage of Human Cytomegalovirus. Preprints 2018, 2018070503. https://doi.org/10.20944/preprints201807.0503.v1
Ourahmane, A.; Cui, X.; He, L.; Dittmer, D.; Schleiss, M.; Hertel, L.; McVoy, M.A. Inclusion of Antibodies to Cell Culture Media Preserves the Integrity of Genes Encoding RL13 and the Pentameric Complex Components during Fibroblast Passage of Human Cytomegalovirus. Preprints2018, 2018070503. https://doi.org/10.20944/preprints201807.0503.v1
APA Style
Ourahmane, A., Cui, X., He, L., Dittmer, D., Schleiss, M., Hertel, L., & McVoy, M.A. (2018). Inclusion of Antibodies to Cell Culture Media Preserves the Integrity of Genes Encoding RL13 and the Pentameric Complex Components during Fibroblast Passage of Human Cytomegalovirus. Preprints. https://doi.org/10.20944/preprints201807.0503.v1
Chicago/Turabian Style
Ourahmane, A., Laura Hertel and Michael A. McVoy. 2018 "Inclusion of Antibodies to Cell Culture Media Preserves the Integrity of Genes Encoding RL13 and the Pentameric Complex Components during Fibroblast Passage of Human Cytomegalovirus" Preprints. https://doi.org/10.20944/preprints201807.0503.v1
Abstract
Propagation of human cytomegalovirus (CMV) in cultured cells results in genetic adaptations that confer improved growth in vitro and significant attenuation in vivo. Mutations in RL13 arise quickly during cell culture passage, while mutations in the UL128-131A locus emerge later during fibroblast passage and disrupt expression of a glycoprotein complex that is important for entry into epithelial and endothelial cells. As in vivo CMV replicates in the context of host antibodies, we reasoned that antibodies might mitigate the accumulation of adaptive mutations during cell culture passage. To test this, CMV in infant urine was used to infect replicate fibroblast cultures. One lineage was passaged in the absence of CMV-hyperimmuneglobulin (HIG) while the other was passaged with HIG in the culture medium. The former lost epithelial tropism and aquired mutations disrupting RL13 and UL131A expression, whereas the latter retained epithelial tropism and both gene loci remained intact after 22 passages. An epitheliotropic RL13+/ UL131A+ virus was isolated by limiting-dilution in the presence of HIG and expanded to produce a working stock sufficient to conduct cell tropism experiments. Thus, culture in the presence of antibodies may facilitate in vitro experiments using viruses that are genetically more authentic than has been previously possible.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
