Preprint Article Version 1 This version is not peer-reviewed

A Virus in American Blackcurrant (Ribes americanum) with Distinct Genome Features Reshapes Classification in the Tymovirales

Version 1 : Received: 21 April 2018 / Approved: 23 April 2018 / Online: 23 April 2018 (10:37:14 CEST)

How to cite: Thekke-Veetil, T.; Ho, T.; Postman, J.D.; Martin, R.R.; Tzanetakis, I.E. A Virus in American Blackcurrant (Ribes americanum) with Distinct Genome Features Reshapes Classification in the Tymovirales. Preprints 2018, 2018040279 (doi: 10.20944/preprints201804.0279.v1). Thekke-Veetil, T.; Ho, T.; Postman, J.D.; Martin, R.R.; Tzanetakis, I.E. A Virus in American Blackcurrant (Ribes americanum) with Distinct Genome Features Reshapes Classification in the Tymovirales. Preprints 2018, 2018040279 (doi: 10.20944/preprints201804.0279.v1).

Abstract

A novel virus with distinct genome features was discovered by high throughput sequencing in a symptomatic blackcurrant plant. The virus tentatively named as blackcurrant virus A (BCVA) has distinct genome organization and molecular features bridging genera in the order Tymovirales. The genome consists of 7106 nucleotides excluding the poly(A) tail. Five open reading frames were identified with the first encoding a putative viral replicase with methyl transferase (MTR), AlkB, helicase and RNA dependent RNA polymerase (RdRp) domains. The other four putative proteins exhibit no significant homology to other virus proteins. The genome organization downstream of the replicase resembles that of members of the order Tymovirales with an unconventional triple gene block (TGB) movement protein arrangement. Phylogenetic analysis using replicase conserved motifs loosely placed BCVA within the Betaflexiviridae whereas it was evolutionarily distant to existing members of the family when using the putative TGBp 1-like and coat protein sequences. Our analysis strongly suggests that BCVA is a novel virus that should be classified as a species in a new genus in the Betaflexiviridae or a new family in the order Tymovirales.

Subject Areas

Betaflexiviridae; blackcurrant; blackcurrant virus A; characterization; detection

Readers' Comments and Ratings (0)

Leave a public comment
Send a private comment to the author(s)
Rate this article
Views 0
Downloads 0
Comments 0
Metrics 0
Leave a public comment

×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.