Version 1
: Received: 9 April 2018 / Approved: 9 April 2018 / Online: 9 April 2018 (10:34:28 CEST)
How to cite:
Choi, K.S.; Jeong, K.S.; Ha, Y.; Choi, K. Complete Chloroplast Genome Sequences of Clematis: IR Expansion and Relative Rates of Synonymous Substitutions. Preprints2018, 2018040106. https://doi.org/10.20944/preprints201804.0106.v1
Choi, K.S.; Jeong, K.S.; Ha, Y.; Choi, K. Complete Chloroplast Genome Sequences of Clematis: IR Expansion and Relative Rates of Synonymous Substitutions. Preprints 2018, 2018040106. https://doi.org/10.20944/preprints201804.0106.v1
Choi, K.S.; Jeong, K.S.; Ha, Y.; Choi, K. Complete Chloroplast Genome Sequences of Clematis: IR Expansion and Relative Rates of Synonymous Substitutions. Preprints2018, 2018040106. https://doi.org/10.20944/preprints201804.0106.v1
APA Style
Choi, K.S., Jeong, K.S., Ha, Y., & Choi, K. (2018). Complete Chloroplast Genome Sequences of <em>Clematis</em>: IR Expansion and Relative Rates of Synonymous Substitutions. Preprints. https://doi.org/10.20944/preprints201804.0106.v1
Chicago/Turabian Style
Choi, K.S., Young-Ho Ha and Kyung Choi. 2018 "Complete Chloroplast Genome Sequences of <em>Clematis</em>: IR Expansion and Relative Rates of Synonymous Substitutions" Preprints. https://doi.org/10.20944/preprints201804.0106.v1
Abstract
Genus Clematis is one of the largest within Ranunculaceae. Here we report the chloroplast genome of two Clematis species, C. brachyura and C. trichotoma endemic to Korea. The chloroplast genome lengths of C. brachyura and C. trichotoma are 159,532 bp and 159,170 bp, respectively. Gene contents in the complete chloroplast genomes of these two Clematis species are identical to that of most Ranunculaceae and other angiosperms. However, our data results demonstrated that genus Clematis has inversion and rearrangement events concerning gene rps4 gene, rps16 to trnH region, and trnL to ndhC region, and IR regions expansion. Comparison of IR regions among Ranunculaceae species revealed that Clematis species contained six protein coding genes (infA, rps8, rpl14, rpl16, rps3, and rpl22) usually found in the long single copy (LSC) region of other species. Phylogenetic analysis demonstrated that genus Clematis is closely related to genus Ranunculus. Differences in repeat structure, substitution rates, and IR expansion in genera Clematis and Ranunculus, explained their relationship. Clematis species showed slightly higher tandem repeats content than Ranunculus species. The six protein-coding genes showed lower synonymous substitution rates in the IR of Clematis species than in the LSC of Ranunculus species. Overall, the chloroplast genomes and results presented here provide important information on the evolution of Ranunculaceae.
Keywords
Clematis; chloroplast genome; rearrangement; inversion; IR expansion; synonymous substitution rate
Subject
Biology and Life Sciences, Plant Sciences
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
