Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Cigarette Smoking Promotes Infection of Cervical Cells by High-Risk Human Papillomaviruses, but not Subsequent E7 Oncoprotein Expression

Kimon Chatzistamatiou
* ORCID logo ,
Theodoros Moysiadis
,
Dimos Vryzas
,
Ekaterini Chatzaki
,
Andreas M. Kaufmann
,
Isabel Koch
,
Erwin Soutschek
,
Oliver Boecher
,
Athena Tsertanidou
,
Nikolaos Maglaveras
,
Pidder Jansen-Duerr
,
Theodoros Agorastos
Version 1 : Received: 5 January 2018 / Approved: 5 January 2018 / Online: 5 January 2018 (11:48:47 CET)

A peer-reviewed article of this Preprint also exists.

Chatzistamatiou, K.; Moysiadis, T.; Vryzas, D.; Chatzaki, E.; Kaufmann, A.M.; Koch, I.; Soutschek, E.; Boecher, O.; Tsertanidou, A.; Maglaveras, N.; Jansen-Duerr, P.; Agorastos, T. Cigarette Smoking Promotes Infection of Cervical Cells by High-Risk Human Papillomaviruses, but not Subsequent E7 Oncoprotein Expression. Int. J. Mol. Sci. 2018, 19, 422. Chatzistamatiou, K.; Moysiadis, T.; Vryzas, D.; Chatzaki, E.; Kaufmann, A.M.; Koch, I.; Soutschek, E.; Boecher, O.; Tsertanidou, A.; Maglaveras, N.; Jansen-Duerr, P.; Agorastos, T. Cigarette Smoking Promotes Infection of Cervical Cells by High-Risk Human Papillomaviruses, but not Subsequent E7 Oncoprotein Expression. Int. J. Mol. Sci. 2018, 19, 422.

Abstract

Persistent cervical infection with high-risk Human papillomaviruses (hrHPVs) is a necessary, but not sufficient, condition for the development of cervical cancer. Therefore, there are other co-factors facilitating the hrHPV carcinogenic process, one of which is smoking. In order to assess the effect of smoking on high-risk (hr) HPV DNA positivity and on the expression of HPV E7 oncoprotein, as a surrogate of persistent hrHPV infection, we used data from women recruited for the PIPAVIR project, which examined the role of E7 protein detection in cervical cancer screening. Women were tested for hrHPV DNA, using Multiplex Genotyping and E7 protein, using a novel sandwich ELISA method, and gave information on their smoking habits. Among 1473 women, hrHPV prevalence was 19.1%. The odds ratio (OR) for hrHPV positivity of smokers compared to non-smokers was 1.785 (95%CI: 1.365-2.332, p<0.001). The ORs for E7 positivity, concerning hrHPV positive women, ranged from 0.720 to 1.360 depending on the E7 detection assay used, but this was not statistically significant. Smoking increases the probability of hrHPV infection, and smoking intensity is positively associated to this increase. Smoking is not related to an increased probability of E7 protein positivity for hrHPV positive women.

Keywords

high-risk HPV infection; E7 oncoprotein; cigarette smoking; HPV carcinogenesis; cervical cancer

Subject

Medicine and Pharmacology, Obstetrics and Gynaecology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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