Preprint Article Version 1 This version is not peer-reviewed

A Novel Mesoporous Multi-Template Molecularly Imprinted Polymer for Selective Separation and Determination of Panax notoginseng Saponins Simultaneously in Biological Samples

Version 1 : Received: 10 November 2017 / Approved: 14 November 2017 / Online: 14 November 2017 (04:47:13 CET)

A peer-reviewed article of this Preprint also exists.

Sun, C.; Wang, J.; Huang, J.; Yao, D.; Wang, C.-Z.; Zhang, L.; Hou, S.; Chen, L.; Yuan, C.-S. The Multi-Template Molecularly Imprinted Polymer Based on SBA-15 for Selective Separation and Determination of Panax notoginseng Saponins Simultaneously in Biological Samples. Polymers 2017, 9, 653. Sun, C.; Wang, J.; Huang, J.; Yao, D.; Wang, C.-Z.; Zhang, L.; Hou, S.; Chen, L.; Yuan, C.-S. The Multi-Template Molecularly Imprinted Polymer Based on SBA-15 for Selective Separation and Determination of Panax notoginseng Saponins Simultaneously in Biological Samples. Polymers 2017, 9, 653.

Journal reference: Polymers 2017, 9, 653
DOI: 10.3390/polym9120653

Abstract

The feasible, reliable and selective multi-template molecularly imprinted polymers (MT-MIPs) based on SBA-15 (SBA-15@MT-MIPs) for the selective separation and determination of the trace level of ginsenoside Rb1 (Rb1), ginsenoside Rg1 (Rg1) and notoginsenoside R1 (R1) (Panax notoginseng saponins, PNS) from biological samples were developed. The polymers were constructed by SBA-15 as support, Rb1, Rg1, R1 as multi-template, acrylamide (AM) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linker. The new synthetic SBA-15@MT-MIPs were satisfactorily applied to solid-phase extraction (SPE) coupled with high performance liquid chromatography (HPLC) for the separation and determination of trace PNS in plasma samples. Under the optimized conditions, the limits of detection (LODs) and quantitation (LOQs) of the proposed method for Rb1, Rg1 and R1 were in the range of 0.63-0.75 ng mL-1 and 2.1-2.5 ng mL-1, respectively. The recoveries of R1, Rb1 and Rg1 were obtained between 93.4% and 104.3% with relative standard deviations (RSDs) in the range of 3.3-4.2%. All results show that the obtained SBA-15@MT-MIPs could be a promising prospect for the practical application in the selective separation and enrichment of trace PNS in the biological samples.

Subject Areas

multi-template molecularly imprinted polymers; SBA-15; Panax notoginseng saponins; separation and determination; solid-phase extraction

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