Preprint Article Version 1 This version not peer reviewed

Antifungal Activity and Action Mode of Cuminic Acid from the Seed of Cuminum cyminum. L against Fusarium oxysporum f. sp. Niveum (FON) on Watermelon

Version 1 : Received: 30 October 2017 / Approved: 30 October 2017 / Online: 30 October 2017 (15:46:36 CET)

How to cite: Sun, Y.; Wang, Y.; Han, L.; Zhang, X.; Feng, J. Antifungal Activity and Action Mode of Cuminic Acid from the Seed of Cuminum cyminum. L against Fusarium oxysporum f. sp. Niveum (FON) on Watermelon. Preprints 2017, 2017100184 (doi: 10.20944/preprints201710.0184.v1). Sun, Y.; Wang, Y.; Han, L.; Zhang, X.; Feng, J. Antifungal Activity and Action Mode of Cuminic Acid from the Seed of Cuminum cyminum. L against Fusarium oxysporum f. sp. Niveum (FON) on Watermelon. Preprints 2017, 2017100184 (doi: 10.20944/preprints201710.0184.v1).

Abstract

Watermelon fusarium wilt caused by Fusarium oxysporum f.sp. niveum (FON) is a destructive soil-borne disease throughout the world leading to serious economic losses and limit watermelon production. Cuminic acid, extracted from the seed of Cuminum cyminum L., belongs to benzoic acid analogues. In this study, the median effective concentration (EC50) values for cuminic acid in inhibiting mycelial growth of FON was 22.53μg/mL. After treatment with cuminic acid, mycelial morphology was seriously influenced; cell membrane permeability and glycerol content were increased markedly, but pigment and mycotoxin (mainly fusaric acid) were significantly decreased. Synthesis genes of bikaverin and fusaric acid both were down regulated compared with the control confirmed by quantitative RT-PCR. In greenhouse experiments, cuminic acid at all concentrations displayed significant bioactivities against FON. Importantly, significant enhancement of activities of SOD, POD, CAT and decrease of MDA content after cuminic acid treatment in watermelon leaves were observed in vivo. These indicated that cuminic acid not only showed high antifungal activity, but also could enhance the self-defense system of the host plant. Above all, cuminic acid showed the potential as a biofungicide to control FON.

Subject Areas

Fusarium oxysporum f. sp. Niveum; p-isopropyl benzoic acid; Biofungicide; Disease management

Readers' Comments and Ratings (1)

Comment 1
Received: 17 February 2018
The commenter has declared there is no conflict of interests.
Comment: In this study, the authors determined the efficacy of cummic acid as an antifungal. Considering the environmental and human health challenges associated with chemical fungicides, developing an antifungal biopesticides is of great significant.

General Comments

The Introduction was well structured and offered sufficient background for the study. The purpose of the study was clear.

Specific Comments

Line 47: please, clarify what you mean by 'for many other pathogens.' You might want to restructure the sentence to improve clarity.
Line 48-50: is this a personal opinion or scientific fact? Most studies recommend pesticide stewardship or regulated use of fungicides.
Line 51: you may want to show that not all fungicides caused the observed drastic effects in soil biodiversity.
Line 52-53: you may want to restructure the sentence to improve clarity.
Line 56-57: remove the remainder of the sentence starting from 'derived' because you have already mentioned in previous paragraph what a fungicide does.
Line 60-62: include references.
Line 238: remove 'the finally used concentration'
Line 253: you may want to state the equipment you used to determine the relative conductivity.
Line 264-273: avoid using the expression 'the described method' and simply describe the method.
Line 274: you may want to clarify that you measure the concentration of mycotoxins rather than 'content of mycotoxin production'. The first former is a mycotoxin level and the later is a rate.
Line 284-285: to improve clarity, avoid using passive tenses. What is OD268?
=== Language and Style ==='
Line 31: Put capital letter C for China
Line 31-32: replace 'has been increased year by year' with 'continues to increase'
Line 32-33: replace ', but' with a period. Start a new sentence.
Line 37: replace 'difficult to be eliminated from' with 'difficult to eliminate in'.
Line 37: remove has
Line 45: replace 'due to' with 'since'
Line 46: replace 'just because of this' with 'as a result'
Line 57-58: replace 'many researches has been reported' with 'several studies found' and add more references to show that they're indeed several studies that support your argument.
Line 247-248: the authors may want to avoid using passive language and use active tense. For example, change the sentence to 'The plates without cuminic acid where used as the control.'
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