Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

CBFA2T2 is Associated with a Cancer Stem Cell State in Renal Cell Carcinoma

Du-Chu Chen
,
You-de Liang
,
Yi-Ze Wang
,
Chun-Zhi Ai
,
Xin-Xing Zhu
,
Ya-Wei Yan
,
yasmeen saeed
,
Bin Yu
,
Jingying huang
,
Yuxin Gao
,
Jiaqi Liu
,
Yi-zhou jiang
,
Min Liu
,
Demeng Chen
* ORCID logo
Version 1 : Received: 4 August 2017 / Approved: 7 August 2017 / Online: 7 August 2017 (15:22:50 CEST)

How to cite: Chen, D.; Liang, Y.; Wang, Y.; Ai, C.; Zhu, X.; Yan, Y.; saeed, Y.; Yu, B.; huang, J.; Gao, Y.; Liu, J.; jiang, Y.; Liu, M.; Chen, D. CBFA2T2 is Associated with a Cancer Stem Cell State in Renal Cell Carcinoma. Preprints 2017, 2017080023. https://doi.org/10.20944/preprints201708.0023.v1 Chen, D.; Liang, Y.; Wang, Y.; Ai, C.; Zhu, X.; Yan, Y.; saeed, Y.; Yu, B.; huang, J.; Gao, Y.; Liu, J.; jiang, Y.; Liu, M.; Chen, D. CBFA2T2 is Associated with a Cancer Stem Cell State in Renal Cell Carcinoma. Preprints 2017, 2017080023. https://doi.org/10.20944/preprints201708.0023.v1

Abstract

Renal cell carcinoma (RCC) is the most common kidney cancer, accounting for approximately 80–90 % of all primary kidney cancer. Treatment for patients with advanced RCC remains unsatisfactory. Rare cancer stem cells (CSCs) are proposed to be responsible for failure of current treatment. Here, we investigate the effects of CBFA2T2 on CSCs regulation in RCC, as well as to elucidate the possible mechanisms. We showed that CBFA2T2 expression can significantly predict the survival of RCC patients. Functional assays showed that knocking-down of CBFA2T2 can inhibit cell migration and invasion in 786-O and A-498 cells in vitro, and reduce ALDHhigh CSCs populations. Furthermore, CBFA2T2 expression is necessary for sphere-forming ability and cancer stem cells marker expression in RCC cell lines. Collectively, our data suggest that CBFA2T2 expression correlates with aggressive characteristics of RCC and CBFA2T2 is required for maintenance of “stemness” through regulation of stem cells factors, thereby highlighting CBFA2T2 as a potential therapeutic target for RCC treatment.

Keywords

CBFA2T2; renal cell carcinoma; cancer stem cells; OCT-4; NANOG

Subject

Medicine and Pharmacology, Oncology and Oncogenics

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.