Preprint Article Version 1 This version is not peer-reviewed

CBFA2T2 is Associated with a Cancer Stem Cell State in Renal Cell Carcinoma

Version 1 : Received: 4 August 2017 / Approved: 7 August 2017 / Online: 7 August 2017 (15:22:50 CEST)

How to cite: Chen, D.; Liang, Y.; Wang, Y.; Ai, C.; Zhu, X.; Yan, Y.; saeed, Y.; Yu, B.; huang, J.; Gao, Y.; Liu, J.; jiang, Y.; Liu, M.; Chen, D. CBFA2T2 is Associated with a Cancer Stem Cell State in Renal Cell Carcinoma. Preprints 2017, 2017080023 (doi: 10.20944/preprints201708.0023.v1). Chen, D.; Liang, Y.; Wang, Y.; Ai, C.; Zhu, X.; Yan, Y.; saeed, Y.; Yu, B.; huang, J.; Gao, Y.; Liu, J.; jiang, Y.; Liu, M.; Chen, D. CBFA2T2 is Associated with a Cancer Stem Cell State in Renal Cell Carcinoma. Preprints 2017, 2017080023 (doi: 10.20944/preprints201708.0023.v1).

Abstract

Renal cell carcinoma (RCC) is the most common kidney cancer, accounting for approximately 80–90 % of all primary kidney cancer. Treatment for patients with advanced RCC remains unsatisfactory. Rare cancer stem cells (CSCs) are proposed to be responsible for failure of current treatment. Here, we investigate the effects of CBFA2T2 on CSCs regulation in RCC, as well as to elucidate the possible mechanisms. We showed that CBFA2T2 expression can significantly predict the survival of RCC patients. Functional assays showed that knocking-down of CBFA2T2 can inhibit cell migration and invasion in 786-O and A-498 cells in vitro, and reduce ALDHhigh CSCs populations. Furthermore, CBFA2T2 expression is necessary for sphere-forming ability and cancer stem cells marker expression in RCC cell lines. Collectively, our data suggest that CBFA2T2 expression correlates with aggressive characteristics of RCC and CBFA2T2 is required for maintenance of “stemness” through regulation of stem cells factors, thereby highlighting CBFA2T2 as a potential therapeutic target for RCC treatment.

Subject Areas

CBFA2T2; renal cell carcinoma; cancer stem cells; OCT-4; NANOG

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