Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Histological and Immunohistochemical Analyses of Repair of the Disc in the Rabbit Temporomandibular Joint Using a Collagen Template

Kuo-Hwa Wang
,
Wing P. Chan
ORCID logo ,
Li-Hsuan Chiu
,
Yu-Hui Tsai
,
Chia-Lang Fang
,
Charn-Bing Yang
,
Hung-Li Tsai
,
Wen-Fu Lai
*
Version 1 : Received: 7 July 2017 / Approved: 11 July 2017 / Online: 11 July 2017 (16:23:01 CEST)

A peer-reviewed article of this Preprint also exists.

Wang, K.-H.; Chan, W.P.; Chiu, L.-H.; Tsai, Y.-H.; Fang, C.-L.; Yang, C.-B.; Chen, K.-C.; Tsai, H.-L.; Lai, W.-F. Histological and Immunohistochemical Analyses of Repair of the Disc in the Rabbit Temporomandibular Joint Using a Collagen Template. Materials 2017, 10, 924. Wang, K.-H.; Chan, W.P.; Chiu, L.-H.; Tsai, Y.-H.; Fang, C.-L.; Yang, C.-B.; Chen, K.-C.; Tsai, H.-L.; Lai, W.-F. Histological and Immunohistochemical Analyses of Repair of the Disc in the Rabbit Temporomandibular Joint Using a Collagen Template. Materials 2017, 10, 924.

Abstract

Previous study demonstrated the reconstituted type I collagen matrix extracted from rabbit tendons enabled to regenerate the TMJ disc in the rabbit. The aim of this study was to investigate changes in the extracellular matrix (ECM) and mechanisms of regeneration in TMJ disc. In 36 New Zealand rabbits that underwent a partial discectomy, discs were replaced with reconstituted collagen templates for 3 months. A histological analysis showed that moderate to severe degeneration appeared in partially discectomized joints without implantation. In contrast, discs that received the reconstituted collagen template regenerated, and returned to normal to protect the joint. Cells in the regenerative tissue expressed ECM, and fibers became regular and compact due to tissue remodeling over time. Reparative cells differentiated into chondroblasts, and showed highly dense pericellular fibers. The morphology and collagen composition of the disc and condyle in the 3-month experimental group were similar to those of normal tissues. In conclusion, the reconstituted collagen template facilitated the regeneration of surgically discectomized discs. Type I and type II collagens play a crucial role in the regeneration of articular discs.

Keywords

temporomandibular joint disc; reconstituted collagen template; tissue regeneration

Subject

Chemistry and Materials Science, Biomaterials

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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