Preprint Brief Report Version 1 This version not peer reviewed

AS1411 Aptamer Conjugated Gelatin-Siloxane Nanoparticles for targeted siRNA delivery

Version 1 : Received: 27 March 2017 / Approved: 27 March 2017 / Online: 27 March 2017 (17:05:36 CEST)

How to cite: Zhao, X.; Tao, S.; Zhang, Y.; Wang, D.; Ren, L. AS1411 Aptamer Conjugated Gelatin-Siloxane Nanoparticles for targeted siRNA delivery. Preprints 2017, 2017030205 (doi: 10.20944/preprints201703.0205.v1). Zhao, X.; Tao, S.; Zhang, Y.; Wang, D.; Ren, L. AS1411 Aptamer Conjugated Gelatin-Siloxane Nanoparticles for targeted siRNA delivery. Preprints 2017, 2017030205 (doi: 10.20944/preprints201703.0205.v1).

Abstract

RNA interference (RNAi) has potential advantages over other gene therapy approaches due to its high specificity and the ability to inhibit target gene expression. Thus, developing a stable, safe, effective siRNA delivery system is necessary to achieve targeted therapy. Here, we developed such a system by conjugating gelatin-based nanoparticles with the nucleolin-targeted AS1411 aptamer and siRNA sequence together (Apt-GS/siRNA NPs) via a disulphide linker. These Apt-GS/siRNA NPs demonstrated favourable release of siRNA under reducing conditions owing to disulphide cleavage. Furthermore, the Apt-GS/siRNA NPs could selectively deliver siRNA to A549 cells overexpressing nucleolin via a nucleolin-mediated transmembrane process, subsequently resulting in efficient RNAi. These results indicated that the Apt-GS/siRNA NPs could facilitate nucleolin-targeted siRNA delivery and gene silencing in tumours.

Subject Areas

siRNA delivery; aptamer AS1411; nucleolin targeting; gene therapy; RNA interference

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