Version 1
: Received: 27 May 2016 / Approved: 27 May 2016 / Online: 27 May 2016 (11:25:30 CEST)
How to cite:
Zhang, N.; Wang, Y.; Xu, W.; Hu, Y.; Ding, J. Poly(lactide-co-glycolide)/Hydroxyapatite Porous Scaffold with Microchannels for Bone Regeneration. Preprints2016, 2016050003. https://doi.org/10.20944/preprints201605.0003.v1
Zhang, N.; Wang, Y.; Xu, W.; Hu, Y.; Ding, J. Poly(lactide-co-glycolide)/Hydroxyapatite Porous Scaffold with Microchannels for Bone Regeneration. Preprints 2016, 2016050003. https://doi.org/10.20944/preprints201605.0003.v1
Zhang, N.; Wang, Y.; Xu, W.; Hu, Y.; Ding, J. Poly(lactide-co-glycolide)/Hydroxyapatite Porous Scaffold with Microchannels for Bone Regeneration. Preprints2016, 2016050003. https://doi.org/10.20944/preprints201605.0003.v1
APA Style
Zhang, N., Wang, Y., Xu, W., Hu, Y., & Ding, J. (2016). Poly(lactide-<em>co</em>-glycolide)/Hydroxyapatite Porous Scaffold with Microchannels for Bone Regeneration. Preprints. https://doi.org/10.20944/preprints201605.0003.v1
Chicago/Turabian Style
Zhang, N., Yong Hu and Jianxun Ding. 2016 "Poly(lactide-<em>co</em>-glycolide)/Hydroxyapatite Porous Scaffold with Microchannels for Bone Regeneration" Preprints. https://doi.org/10.20944/preprints201605.0003.v1
Abstract
Mass transfer restrictions of scaffolds are currently hindering the development of three-dimensional (3D), clinically viable, and tissue engineered constructs. For this situation, a 3D poly(lactide-co-glycolide)/hydroxyapatite porous scaffold, which was much favorable for transfer of nutrients to and waste products from the cells in the pores, was developed in this study. The 3D scaffold had an innovative structure, including macropores with diameters of 300−450 μm for cell ingrowth and microchannels with diameters of 2−4 μm for nutrition and waste exchange. The mechanical strength in wet state was strong enough to offer the structural support. The typical structure was more beneficial for the attachment, proliferation, and differentiation of rabbit bone marrow mesenchymal stem cells (rBMSCs). The alkaline phosphatase (ALP) activity and calcium (Ca) deposition were evaluated on the differentiation of rBMSCs, and the results indicated that the microchannel structure was very favorable for differentiating rBMSCs into maturing osteoblasts. For repairing rabbit radius defects in vivo, there was rapid healing in the defects treated with the 3D porous scaffold with microchannels, where the bridging by a large bony callus was observed at 12 weeks post-surgery. Based on the results, the 3D porous scaffold with microchannels was a promising candidate for bone defect repair.
Keywords
poly(lactide-co-glycolide); hydroxyapatite; porous scaffold; microchannel; cell ingrowth; mass exchange; bone tissue engineering
Subject
Chemistry and Materials Science, Polymers and Plastics
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
