Version 1
: Received: 14 May 2024 / Approved: 14 May 2024 / Online: 15 May 2024 (10:31:08 CEST)
How to cite:
Lopes, S. C.; Leocadio, P. C. L.; Ciurleo, G. C. V.; Matos, G. A.; Souza, M. M. D.; de Paiva, M. J. N.; Zandonadi, F.; Sussulini, A.; Raposo, R. D. S.; da Silva, F. L.; Zampieri, D.; Martins, A. M. A.; Leite, J. I. A.; Oriá, R. B. Methylmercury Intoxication Affects Epididymal White Adipose Tissue and Liver Function in Young ApoE Knockout Mice. Preprints2024, 2024050993. https://doi.org/10.20944/preprints202405.0993.v1
Lopes, S. C.; Leocadio, P. C. L.; Ciurleo, G. C. V.; Matos, G. A.; Souza, M. M. D.; de Paiva, M. J. N.; Zandonadi, F.; Sussulini, A.; Raposo, R. D. S.; da Silva, F. L.; Zampieri, D.; Martins, A. M. A.; Leite, J. I. A.; Oriá, R. B. Methylmercury Intoxication Affects Epididymal White Adipose Tissue and Liver Function in Young ApoE Knockout Mice. Preprints 2024, 2024050993. https://doi.org/10.20944/preprints202405.0993.v1
Lopes, S. C.; Leocadio, P. C. L.; Ciurleo, G. C. V.; Matos, G. A.; Souza, M. M. D.; de Paiva, M. J. N.; Zandonadi, F.; Sussulini, A.; Raposo, R. D. S.; da Silva, F. L.; Zampieri, D.; Martins, A. M. A.; Leite, J. I. A.; Oriá, R. B. Methylmercury Intoxication Affects Epididymal White Adipose Tissue and Liver Function in Young ApoE Knockout Mice. Preprints2024, 2024050993. https://doi.org/10.20944/preprints202405.0993.v1
APA Style
Lopes, S. C., Leocadio, P. C. L., Ciurleo, G. C. V., Matos, G. A., Souza, M. M. D., de Paiva, M. J. N., Zandonadi, F., Sussulini, A., Raposo, R. D. S., da Silva, F. L., Zampieri, D., Martins, A. M. A., Leite, J. I. A., & Oriá, R. B. (2024). Methylmercury Intoxication Affects Epididymal White Adipose Tissue and Liver Function in Young ApoE Knockout Mice. Preprints. https://doi.org/10.20944/preprints202405.0993.v1
Chicago/Turabian Style
Lopes, S. C., Jacqueline Isaura A. Leite and Reinaldo B. Oriá. 2024 "Methylmercury Intoxication Affects Epididymal White Adipose Tissue and Liver Function in Young ApoE Knockout Mice" Preprints. https://doi.org/10.20944/preprints202405.0993.v1
Abstract
Methylmercury (MeHg) intoxication is a global health concern. Although MeHg neurotoxicity is well-recognized, less is known about MeHg's effects on metabolism. In this study, we evaluated MeHg intoxication (20mg/L in drinking water for 20 days) effects on liver and epididymal white fat (EWF) in young ApoE knockout (ko) and wild-type mice. The former is well-known to show spontaneous dyslipidemia. Hg levels in hair, liver, and EWF were measured to assess the degree of MeHg exposure. We also evaluated body weight gain, plasma triglycerides, total cholesterol levels, and liver injury by assessing steatosis score, AST, ALT, SOD, and TBAR levels. To evaluate EWF, we analyzed mean wet weight and adipocyte diameter, plasma leptin levels and metabolomics. Hg levels were markedly higher among intoxicated mice. ApoE ko mice showed higher Hg in the hair but lower levels in the liver and EWF than wild-type controls. Among wild-type mice, MeHg compromised weight gain and increased liver TBAR levels compared to non-challenged controls. Among non-intoxicated mice, ApoE deficiency significantly increased triglyceride, total cholesterol, and liver transaminases with reduced EWF wet weight, hepatic SOD, and plasma leptin levels. MeHg accumulation together with ApoE deficiency elevated total cholesterol, triglycerides, hepatic transaminases, and TBARS. We found distinct EWF metabolite activity in different scenarios of ApoE deficiency and MeHg intoxication, highlighting increased cardiovascular risk especially when both challenges occur. Further studies are required to elucidate the underlying mechanisms involved in these effects and the target nutritional interventions in most need to ameliorate them.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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