Version 1
: Received: 5 January 2024 / Approved: 5 January 2024 / Online: 8 January 2024 (16:22:14 CET)
How to cite:
Gómez-Blanco, J. C.; Macías-García, A.; Rodríguez Rego, J. M.; Mendoza Cerezo, L.; Sanchez-Margallo, F. M.; Marcos Romero, A. C.; Pagador, J. B. Combination of Review and Design of Experiments to Optimize Computational Simulations on Bioprinting Nozzles. Preprints2024, 2024010617. https://doi.org/10.20944/preprints202401.0617.v1
Gómez-Blanco, J. C.; Macías-García, A.; Rodríguez Rego, J. M.; Mendoza Cerezo, L.; Sanchez-Margallo, F. M.; Marcos Romero, A. C.; Pagador, J. B. Combination of Review and Design of Experiments to Optimize Computational Simulations on Bioprinting Nozzles. Preprints 2024, 2024010617. https://doi.org/10.20944/preprints202401.0617.v1
Gómez-Blanco, J. C.; Macías-García, A.; Rodríguez Rego, J. M.; Mendoza Cerezo, L.; Sanchez-Margallo, F. M.; Marcos Romero, A. C.; Pagador, J. B. Combination of Review and Design of Experiments to Optimize Computational Simulations on Bioprinting Nozzles. Preprints2024, 2024010617. https://doi.org/10.20944/preprints202401.0617.v1
APA Style
Gómez-Blanco, J. C., Macías-García, A., Rodríguez Rego, J. M., Mendoza Cerezo, L., Sanchez-Margallo, F. M., Marcos Romero, A. C., & Pagador, J. B. (2024). Combination of Review and Design of Experiments to Optimize Computational Simulations on Bioprinting Nozzles. Preprints. https://doi.org/10.20944/preprints202401.0617.v1
Chicago/Turabian Style
Gómez-Blanco, J. C., Alfonso Carlos Marcos Romero and J. Blas Pagador. 2024 "Combination of Review and Design of Experiments to Optimize Computational Simulations on Bioprinting Nozzles" Preprints. https://doi.org/10.20944/preprints202401.0617.v1
Abstract
3D bioprinting, like 3D printing, is a process that builds structures by depositing material layer by layer. In the specific case of bioprinting, these layers are composed of a biocompatible material together with living cells, allowing the creation of three-dimensional structures that recreate functional tissues and organs. Bioprinting cell-laden structures is complicated by the high rate of cell damage and stress that occurs during the process, caused by the high pressures and stresses to which they are subjected. To minimise damage during the process, it is important to study and optimise certain bioprinting conditions beforehand and to analyse how they affect the cells by means of computational simulations. In this review, both quantitative and qualitative data are collected to improve the nozzle geometry through computational simulation studies. Optimal ranges for nozzle diameter (0.2-1mm) and length (8-10mm; 300-900μm) have been defined, and recommendations for improving nozzle performance during bioprinting, such as the provision of an inner angle of 20-30°, an inner EDTA coating and a shear stress of less than 10kPa, have been gathered. Finally, based on the data collected, a design of experiments is proposed to obtain an optimal bioprinting configuration for a particular bioink.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.