Version 1
: Received: 7 September 2023 / Approved: 8 September 2023 / Online: 11 September 2023 (04:11:02 CEST)
How to cite:
Park, S.-J.; Jo, M.-J.; Ryu, Y.; Seo, M.-G.; Lee, J. W.; Kim, Y. H.; Kim, Y.; Park, J.-K. Agrimonia pilosa Extract Alleviates CDAHFD-Induced Non-Alcoholic Steatohepatitis and Fibrosis in Mice. Preprints2023, 2023090608. https://doi.org/10.20944/preprints202309.0608.v1
Park, S.-J.; Jo, M.-J.; Ryu, Y.; Seo, M.-G.; Lee, J. W.; Kim, Y. H.; Kim, Y.; Park, J.-K. Agrimonia pilosa Extract Alleviates CDAHFD-Induced Non-Alcoholic Steatohepatitis and Fibrosis in Mice. Preprints 2023, 2023090608. https://doi.org/10.20944/preprints202309.0608.v1
Park, S.-J.; Jo, M.-J.; Ryu, Y.; Seo, M.-G.; Lee, J. W.; Kim, Y. H.; Kim, Y.; Park, J.-K. Agrimonia pilosa Extract Alleviates CDAHFD-Induced Non-Alcoholic Steatohepatitis and Fibrosis in Mice. Preprints2023, 2023090608. https://doi.org/10.20944/preprints202309.0608.v1
APA Style
Park, S. J., Jo, M. J., Ryu, Y., Seo, M. G., Lee, J. W., Kim, Y. H., Kim, Y., & Park, J. K. (2023). Agrimonia pilosa Extract Alleviates CDAHFD-Induced Non-Alcoholic Steatohepatitis and Fibrosis in Mice. Preprints. https://doi.org/10.20944/preprints202309.0608.v1
Chicago/Turabian Style
Park, S., Yongduk Kim and Jin-Kyu Park. 2023 "Agrimonia pilosa Extract Alleviates CDAHFD-Induced Non-Alcoholic Steatohepatitis and Fibrosis in Mice" Preprints. https://doi.org/10.20944/preprints202309.0608.v1
Abstract
Non-alcoholic steatohepatitis (NASH) is diagnosed by the occurrence of fat buildup, inflammation, and fibrotic changes within the liver. NASH has the capacity to proceed to the advancement of decompensated cirrhosis and carcinoma by inflammation and oxidative stress. Although Agrimonia pilosa has traditionally been used medicinal purposes, its effects on the liver remains incompletely elucidated. In this research, we assessed the therapeutic outcomes of Agrimonia pilosa extract (APE) on free fatty acid (FFA)-treated HepG2 cellular hepatic steatosis and in a choline-deficient, L-amino acid-defined, high-fat diet with 0.1% methionine (CDAHFD)-induced NASH mouse model. In FFA-treated cells, APE inhibited intracellular lipid accumulation, and regulated the mRNA and protein expressions of modulators of fatty acid (FA) synthesis as well as FA oxidation proteins, through down-regulation of the AMP-activated protein kinase/sirtuin 1 signaling pathway. In the CDAHFD-induced NASH mouse model, APE significantly enhanced liver histology and serum levels of liver damage. APE also significantly attenuated hepatic expression of mRNA and proteins related to FA synthesis, inflammation, and fibrosis. Furthermore, APE mitigated the expression levels of oxidative stress and endoplasmic reticulum stress. Overall, these discoveries indicate that Agrimonia pilosa has the potential to treat NASH by regulating hepatic lipid metabolism, alleviating inflammation, and mitigating oxidative stress.
Medicine and Pharmacology, Complementary and Alternative Medicine
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.