Review
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Preserved in Portico This version is not peer-reviewed
New Advances in the Exploration of Esterases wit PET and Fluorescent Probes
Version 1
: Received: 31 July 2023 / Approved: 1 August 2023 / Online: 2 August 2023 (10:07:30 CEST)
A peer-reviewed article of this Preprint also exists.
Gil-Rivas, A.; de Pascual-Teresa, B.; Ortín, I.; Ramos, A. New Advances in the Exploration of Esterases with PET and Fluorescent Probes. Molecules 2023, 28, 6265. Gil-Rivas, A.; de Pascual-Teresa, B.; Ortín, I.; Ramos, A. New Advances in the Exploration of Esterases with PET and Fluorescent Probes. Molecules 2023, 28, 6265.
Abstract
Esterases are hydrolases that catalyze the hydrolysis of esters into the corresponding acids and alcohols. The development of fluorescent probes for detecting esterases is of great importance due to their wide spectrum of biological and industrial applications. These probes can provide a rapid and sensitive method for detecting the presence and activity of esterases in various samples, including biological fluids, food products, and environmental samples. Fluorescent probes can also be used for monitoring the effects of drugs and environmental toxins on esterase activity, as well as to study the functions and mechanisms of these enzymes in several biological systems. Additionally, fluorescent probes can be designed to selectively target specific types of esterases, such as those found in pathogenic bacteria or cancer cells. In this review, we summarize the recent fluorescent probes described for the visualization of cell viability and some applications for in vivo imaging. On the other hand, Positron Emission Tomography (PET) is a nuclear-based molecular imaging modality of great value to study the activity of enzymes in vivo. We provide some examples of PET probes for imaging acetylcholinesterases and butyrylcholinesterases in brain, which are valuable tools for diagnosing dementia and monitoring the effects of anticholinergic drugs on the central nervous system.
Keywords
Positron Emission Tomography (PET); fluorescent probes; esterases; carboxiesterases; acetylcholinesterases; butyrylcholinesterases; neurodegenerative diseases; cancer.
Subject
Chemistry and Materials Science, Medicinal Chemistry
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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