Preprint Article Version 1 NOT YET PEER-REVIEWED

TGF-β and Physiological Root Resorption of Deciduous Teeth

  1. Department of Pediatric Dentistry, School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan
  2. Department of Biochemistry and Molecular Biology, School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan
Version 1 : Received: 27 October 2016 / Approved: 27 October 2016 / Online: 27 October 2016 (12:10:55 CEST)

How to cite: Shimazaki, E.; Karakida, T.; Yamamoto, R.; Kobayashi, S.; Fukae, M.; Yamakoshi, Y.; Asada, Y. TGF-β and Physiological Root Resorption of Deciduous Teeth. Preprints 2016, 2016100121 (doi: 10.20944/preprints201610.0121.v1). Shimazaki, E.; Karakida, T.; Yamamoto, R.; Kobayashi, S.; Fukae, M.; Yamakoshi, Y.; Asada, Y. TGF-β and Physiological Root Resorption of Deciduous Teeth. Preprints 2016, 2016100121 (doi: 10.20944/preprints201610.0121.v1).

Abstract

The present study was performed to examine that transforming growth factor beta (TGF-β) in root-surrounding tissues on deciduous teeth during the physiological root resorption regulates the differentiation induction into odontoclast. We prepared root-surrounding tissues with (R) or without (N) physiological root resorption scraped off at three regions (R1-R3 or N1-N3) from the cervical area to the apical area of the tooth and measured both TGF-β and the tartrate-resistant acid phosphatase (TRAP) activities. The TGF-β activity level was increased in N1-N3, whereas the TRAP activity was increased in R2 and R3. In vitro experiments for RANKL-mediated osteoclast differentiation revealed that TGF-β in N1-N3 and R1-R3 enhanced the TRAP activity in RAW264 cells. A genetic study indicated that the mRNA level of TGF-β1 in N1 and N2 was significantly increased, and corresponded with that of osteoprotegerin (OPG). In contrast, the expression level of receptor activator of NF-κB ligand (RANKL) was increased in R2 and R3. Our findings suggest that TGF-β is closely related to the regulation of OPG induction and RANKL-mediated odontoclast differentiation depending on the timing of RANKL and OPG mRNA expression in the root-surrounding tissues of deciduous teeth during physiological root resorption.

Subject Areas

cytokine; gene expression; osteoclast; root resorption; pediatric dentistry; protein expression

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