Preprint Article Version 1 NOT YET PEER-REVIEWED

Signal-on and Sandwich Electrochemiluminescence Immunosensor for Diethylstilbestrol Based on Magnetic Molecularly Imprinted Polymers as Capture Probe and Quantum Dots Labeled Antibody as Labels

  1. The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Material Science and Chemical Engineering of Ningbo University, Ningbo 315211, China
Version 1 : Received: 14 September 2016 / Approved: 15 September 2016 / Online: 15 September 2016 (06:14:58 CEST)

How to cite: Zhang, L.; Zhang, D.; Gan, N.; Zhou, J.; Li, T.; Cao, Y. Signal-on and Sandwich Electrochemiluminescence Immunosensor for Diethylstilbestrol Based on Magnetic Molecularly Imprinted Polymers as Capture Probe and Quantum Dots Labeled Antibody as Labels. Preprints 2016, 2016090047 (doi: 10.20944/preprints201609.0047.v1). Zhang, L.; Zhang, D.; Gan, N.; Zhou, J.; Li, T.; Cao, Y. Signal-on and Sandwich Electrochemiluminescence Immunosensor for Diethylstilbestrol Based on Magnetic Molecularly Imprinted Polymers as Capture Probe and Quantum Dots Labeled Antibody as Labels. Preprints 2016, 2016090047 (doi: 10.20944/preprints201609.0047.v1).

Abstract

A signal-on and sandwich electrochemiluminescence immunoassay (ECLIA) for diethylstilbestrol (DES) using magnetic surface molecular imprinting polymers (MMIPs) -antibody labeled CdS quantum dots (CdS-Ab) composite probes. Firstly, the MMIPs were synthesized through employing phenol as mimicking template to imprint inside the poly-dopamine film on the surface of Fe3O4@SiO2 (MMIPs) then elute the template by 0.01M HCl. Secondly, the antibody of DES was labeled on the surface of the CdS QDs to form the signal tag (CdS-Ab).When the target, MMIPs and CdS-Ab was mixed together, a sandwich MMIPs-target-CdS-Ab composite was constructed. It can easily absorbed on the surface of a screen printed carbon electrode by external magnetic field and emit electrochemical luminescence signal at potential -0.2V. The intensity depended linearly on the logarithm of DES concentration from 0.1 to 1.0 ×105 pg mL-1, with the detection limit (LOD) of 0.03 pg mL-1 (S/N = 3). Several samples of fish samples were tested based on the immunoassay which showed higher selectivity and good recoveries between 80% to 104%.The method was used to detect DES in fish with consistent results as that of conventional ELISA.

Subject Areas

diethylstilbestrol; signal-on; sandwich electrochemiluminescence immunosensor; magnetic molecularly imprinted polymers; QDs-Ab; fish

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