Preprint Article Version 1 NOT YET PEER-REVIEWED

Prevalence, Characterization and Mycotoxin Production Ability of Fusarium species on Korean Adlay (Coix lacrymal-jobi L.) Seeds

  1. Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science (NIHHS), Eumseong, Chungbuk 27709, Korea
  2. Department of Applied Biology, College of Agriculture & Life Sciences, Chungnam National University, Daejeon 34134, Korea
  3. Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration, Muan 58545, Korea
  4. Laboratory of Mucosal Exposome and Biomodulation, Department of Biomedical Sciences, Pusan National University School of Medicine, Yangsan 50612, Korea
Version 1 : Received: 3 August 2016 / Approved: 4 August 2016 / Online: 4 August 2016 (10:12:54 CEST)

A peer-reviewed article of this Preprint also exists.

An, T.J.; Shin, K.S.; Paul, N.C.; Kim, Y.G.; Cha, S.W.; Moon, Y.; Yu, S.H.; Oh, S.-K. Prevalence, Characterization, and Mycotoxin Production Ability of Fusarium Species on Korean Adlay (Coix lacrymal-jobi L.) Seeds. Toxins 2016, 8, 310. An, T.J.; Shin, K.S.; Paul, N.C.; Kim, Y.G.; Cha, S.W.; Moon, Y.; Yu, S.H.; Oh, S.-K. Prevalence, Characterization, and Mycotoxin Production Ability of Fusarium Species on Korean Adlay (Coix lacrymal-jobi L.) Seeds. Toxins 2016, 8, 310.

Journal reference: Toxins 2016, 8, 310
DOI: 10.3390/toxins8110310

Abstract

Adlay seed samples were collected from 3 adlay growing regions (Yeoncheon, Jeonnam and Eumseong regions) in Korea during 2012. Among all the samples collected, 400 seeds were tested for fungal occurrence by standard blotter and test tube agar methods and different taxonomic groups of fungal genera were detected. The most predominant fungal genera encountered were Fusarium, Phoma, Alternaria, Cladosporium, Curvularia, Cochliobolus and Leptosphaerulina. The occurrence of Fusarium species were 45.6% and based on the combined sequences of two protein coding genes, EF-1a, Beta-tubulin and phylogenetic analysis, 10 species were characterized as F. incarnatum (11.67%), F. kyushense (10.33%), F. fujikuroi (8.67%), F. concentricum (6.00%), F. asiaticum (5.67%), F. graminearum (1.67%), F. miscanthi (0.67%), F. polyphialidiom (0.33%), F. armeniacum (0.33%) and F. thapsinum (0.33%). The ability of these isolates to produce mycotoxins fumonisin (FUM) and zeralenone (ZEN) were tested by ELISA quantitative analysis method. The result revealed that fumonisin (FUM) was produced only by F. fujikuroi and zeralenone (ZEN) by F. asiaticum & F. graminearum. Mycotoxigenic species were then examined for their morphological characteristics to confirm their identity. Morphological observations of the species correlated well with their molecular identification and confirmed as F. asiaticum, F. fujikuroi and F. graminearum.

Subject Areas

seeds; ELISA; Fusarium; morphological data analysis; mycotoxins; phylogenetic analysis S

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